UniProt Accession

 ALKB1_HUMAN; Q13686; Q8TAU1; Q9ULA7 

Genbank Protein ID

 X91992; AC008044; BC025787 

Genbank Nucleotide ID

 CAA63047.1; AAF01478.1; AAH25787.1 

Protein Name

 Alkylated DNA repair protein alkB homolog 1 

Protein Synonyms/Alias

 Alpha-ketoglutarate-dependent dioxygenase ABH1; DNA lyase ABH1; DNA oxidative demethylase ALKBH1 

Gene Name

 ALKBH1 

Gene Synonyms/Alias

 ABH; ABH1; ALKBH 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
87	AGLQPVSKWQAYGLK	ubiquitination	[1]
137	NLDKHMSKEETQDLW	ubiquitination	[2]
148	QDLWEQSKEFLRYKE	ubiquitination	[1]
362	LEPIEDEKRDISTEG	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron. 

Sequence Annotation

 DOMAIN 208 347 Fe2OG dioxygenase.
 REGION 177 179 Substrate binding (By similarity).
 REGION 220 222 Alpha-ketoglutarate binding (By
 REGION 338 344 Alpha-ketoglutarate binding (By
 METAL 231 231 Iron; catalytic (By similarity).
 METAL 233 233 Iron; catalytic (By similarity).
 METAL 287 287 Iron; catalytic (By similarity).
 BINDING 170 170 Substrate (By similarity).
 BINDING 261 261 Substrate (By similarity).  

Keyword

 Complete proteome; Dioxygenase; DNA damage; DNA repair; Iron; Lyase; Metal-binding; Mitochondrion; Multifunctional enzyme; Nucleus; Oxidoreductase; Polymorphism; Reference proteome; RNA repair. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 389 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0005719; C:nuclear euchromatin; IEA:Compara.
 GO:0042056; F:chemoattractant activity; IEA:Compara.
 GO:0003906; F:DNA-(apurinic or apyrimidinic site) lyase activity; IDA:UniProtKB.
 GO:0008198; F:ferrous iron binding; IDA:UniProtKB.
 GO:0070579; F:methylcytosine dioxygenase activity; IDA:UniProtKB.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0048589; P:developmental growth; IEA:Compara.
 GO:0006307; P:DNA dealkylation involved in DNA repair; IDA:UniProtKB.
 GO:0080111; P:DNA demethylation; IDA:UniProtKB.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0001764; P:neuron migration; IEA:Compara.
 GO:0031175; P:neuron projection development; IEA:Compara.
 GO:0070989; P:oxidative demethylation; IDA:UniProtKB.
 GO:0001890; P:placenta development; IEA:Compara.
 GO:0042245; P:RNA repair; IDA:UniProtKB. 

Interpro

 IPR004574; Alkb.
 IPR027450; AlkB-like.
 IPR005123; Oxoglu/Fe-dep_dioxygenase. 

Pfam

 PF13532; 2OG-FeII_Oxy_2 

SMART

  

PROSITE

 PS51471; FE2OG_OXY 

PRINTS