UniProt Accession

 HIBCH_MOUSE; Q8QZS1 

Genbank Protein ID

 AK076038; BC026437 

Genbank Nucleotide ID

 BAC36138.1; AAH26437.1 

Protein Name

 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial 

Protein Synonyms/Alias

 3-hydroxyisobutyryl-coenzyme A hydrolase; HIB-CoA hydrolase; HIBYL-CoA-H 

Gene Name

 Hibch 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
54	VITLNRPKFLNALSL	acetylation	[1]
91	IIKGAGGKAFCAGGD	acetylation	[1]
100	FCAGGDIKALSEAKK	acetylation	[1]
220	THFVDSEKLRVLEEE	acetylation	[1]
249	VLESYHAKSKMDQDK	acetylation	[1]
251	ESYHAKSKMDQDKSI	acetylation	[1]
296	PFAIEQMKVINKMSP	acetylation	[1, 2, 3, 4, 5]
296	PFAIEQMKVINKMSP	succinylation	[4]
300	EQMKVINKMSPTSLK	acetylation	[3, 4]
300	EQMKVINKMSPTSLK	succinylation	[4]
352	VRAVLIDKDQTPKWK	acetylation	[1, 2, 3, 4, 5, 6, 7, 8, 9, 10]
352	VRAVLIDKDQTPKWK	succinylation	[4]
357	IDKDQTPKWKPANLK	acetylation	[1, 4, 5]
357	IDKDQTPKWKPANLK	succinylation	[4]
359	KDQTPKWKPANLKDV	acetylation	[3, 5]
364	KWKPANLKDVTDEDL	acetylation	[3, 4, 5]
364	KWKPANLKDVTDEDL	succinylation	[4]
376	EDLNSYFKSLGSSDL	acetylation	[4, 5]
376	EDLNSYFKSLGSSDL	succinylation	[4]
384	SLGSSDLKF******	acetylation	[3]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [6] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [7] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [8] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [9] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [10] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599] 

Functional Description

 Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (By similarity). 

Sequence Annotation

 BINDING 120 120 Substrate (By similarity).
 BINDING 145 145 Substrate; via amide nitrogen (By
 BINDING 168 168 Substrate (By similarity).
 BINDING 176 176 Substrate (By similarity).
 MOD_RES 91 91 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Branched-chain amino acid catabolism; Complete proteome; Direct protein sequencing; Hydrolase; Mitochondrion; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 385 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0003860; F:3-hydroxyisobutyryl-CoA hydrolase activity; IEA:EC.
 GO:0006574; P:valine catabolic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR014748; Crontonase_C. 

Pfam

  

SMART

  

PROSITE

 PS00166; ENOYL_COA_HYDRATASE 

PRINTS