CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018704
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Exportin-5 
Protein Synonyms/Alias
 Exp5; Ran-binding protein 21 
Gene Name
 Xpo5 
Gene Synonyms/Alias
 Kiaa1291; Ranbp21 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
41LKFCEEFKEKCPICVubiquitination[1]
407ASMTNLVKMGFPSKTubiquitination[1]
413VKMGFPSKTDSPSCEubiquitination[1]
590FESVGESKAPRTRAVubiquitination[1]
610HACSSINKMCRDYPDubiquitination[1]
1160LIAGCIGKPLGEQFRacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi) (By similarity). Mediates nuclear export of ADAR/ADAR1 in a RanGTP-dependent manner (By similarity). 
Sequence Annotation
 REGION 2 108 Necessary for interaction with Ran (By
 REGION 533 640 Necessary for interaction with ILF3 (By
 MOD_RES 396 396 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Nucleus; Protein transport; Reference proteome; RNA-binding; RNA-mediated gene silencing; Transport; tRNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1204 AA 
Protein Sequence
MEMEQVNALC EELVKAVTVM MDPSSTQRYR LEALKFCEEF KEKCPICVPC GLKLAEKTQI 60
AIVRHFGLQI LEHVVKFRWN SMSRLEKVYL KNSVMELIAN GTLRILEEEN HIKDVLSRIV 120
VEMIKREWPQ HWPDMLMELD TLFRQGETQR ELVMFILLRL AEDVVTFQTL PTQRRRDIQQ 180
TLTQNMERIL NFLLNTLQEN VNKYQQMKTD SSQEAEAQAN CRVSVAALNT LAGYIDWVSL 240
NHITAENCKL VETLCLLLNE QELQLGAAEC LLIAVSRKGK LEDRKRLMIL FGDVAMHYIL 300
SAAQTADGGG LVEKHYLFLK RLCQVLCALG NLLCALLALD ANIQTPINFG MYLESFLAFT 360
THPSQFLRSS THMTWGALFR HEVLSRDPAL LAVIPKYLRA SMTNLVKMGF PSKTDSPSCE 420
YSRFDFDSDE DFNAFFNSSR AQHGEVVRCV CRLDPKTSFQ MAAEWLKYQL SASIDTGPVN 480
SCSTAGTGEG GFCSIFSPSY VQWEAMTFFL ESVINQMFRT LDKEELPVSD GIELLQLVLN 540
FEIKDPLVLS CVLTNVSALF PFVTYKPAFL PQVFSKLFSF VTFESVGESK APRTRAVRNV 600
RRHACSSINK MCRDYPDLVL PNFDMLYSHV KQLLSNELLL TQMEKCALME ALVLVSNQFK 660
DYERQKLFLE ELMAPVVNIW LSEEMCRALS DIDSFIAYVG ADLKSCDPAV EDPCGLNRAR 720
MSFCVYSILG VMRRTSWPSD LEEAKAGGFV VGYTPSGNPI FRNPCTEQIL RLLDNLLALV 780
RTHNTLYTPE MLTKMAEPFT KALDIVESEK TAILGLPQPL LEFNDHPVYR TTLERMQRFF 840
GILYENCYHI LGKAGPSMQQ DFYTVEDLAS QLLGSAFVNL NNIPDFRLRS MLRVFVKPLV 900
LFCPSEHYET LISPILGPLF TYLHMRLSQK WHVINQRSIL CGEDEIAEDN PESQEMLEEQ 960
LVRMLTREAM DLIMACCVSK KTADHTAAPT ADGDDEEMMA TEVAPSSVVE LTDLGKCLMK 1020
HEDVCTALLI TAFNSLTWKD TLSCQRATTQ LCWPLLKQVM SGTLLADAVT WLFTSVLKGL 1080
QMHGQHDGCM ASLVHLAFQI YEALRPRYLE IRAVMEQIPE INKESLDQFD CKLLNPSLQK 1140
AADKRRKDHF KRLIAGCIGK PLGEQFRKEV HIKNLPWLFK KPKPMLETEV LDSEEGGLAT 1200
IFEP 1204 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005643; C:nuclear pore; IEA:InterPro.
 GO:0008565; F:protein transporter activity; IDA:MGI.
 GO:0000049; F:tRNA binding; IDA:MGI.
 GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
 GO:0006611; P:protein export from nucleus; IDA:MGI. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR013598; Exportin-1/Importin-b-like.
 IPR001494; Importin-beta_N. 
Pfam
 PF08389; Xpo1 
SMART
 SM00913; IBN_N 
PROSITE
  
PRINTS