CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011448
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 
Protein Synonyms/Alias
 InsP6 and PP-IP5 kinase 
Gene Name
 VIP1 
Gene Synonyms/Alias
 YLR410W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
37MSPLFLNKNTQKAMQacetylation[1]
615VLANALEKKLNFPGTubiquitination[2]
638NKENEVEKVQFILKWacetylation[1]
1086DMDYVQQKLRNKFTRacetylation[1]
1143SSSTSVNKTLD****acetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.
 Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH, Kleifeld O.
 Mol Cell Proteomics. 2011 May;10(5):M111.009753. [PMID: 21427232] 
Functional Description
 Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP- InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. Phosphorylates inositol hexakisphosphate (InsP6) at positions 1 or 3 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates at position 1 or 3 PP-InsP5, produced by IP6Ks from InsP6, to produce (PP)2- InsP4 (By similarity). Required for maintaining cellular integrity, normal growth and interactions with the ARP complex. Acts as a regulator of the PHO80-PHO85 cyclin/cyclin-dependent kinase (CDK) complex, thereby regulating signaling of phosphate availability. Required for the function of the cortical actin cytoskeleton, possibly by participating in correct F-actin localization and ensuring polarized growth. 
Sequence Annotation
 NP_BIND 402 405 ATP (By similarity).
 NP_BIND 412 414 ATP (By similarity).
 NP_BIND 487 489 ATP (By similarity).
 REGION 197 198 Substrate binding (By similarity).
 REGION 377 378 Substrate binding (By similarity).
 REGION 492 495 Substrate binding (By similarity).
 BINDING 278 278 ATP (By similarity).
 BINDING 351 351 ATP (By similarity).
 BINDING 358 358 ATP (By similarity).
 BINDING 377 377 ATP (By similarity).
 BINDING 414 414 Substrate (By similarity).
 BINDING 428 428 Substrate (By similarity).
 BINDING 430 430 ATP (By similarity).
 BINDING 475 475 ATP (By similarity).
 MOD_RES 31 31 Phosphoserine.
 MOD_RES 54 54 Phosphoserine.
 MOD_RES 77 77 Phosphoserine.
 MOD_RES 895 895 Phosphoserine.
 MOD_RES 1107 1107 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1146 AA 
Protein Sequence
MSGIKKEPIE SDEVPQQETK NNLPSAPSEM SPLFLNKNTQ KAMQSIAPIL EGFSPKTSAS 60
ENMSLKLPPP GIQDDHSEEN LTVHDTLQRT ISTALGNGNN TNTVTTSGLK KADSESKSEA 120
DPEGLSNSNI VNDADNINSI SKTGSPHLPQ GTMDAEQTNM GTNSVPTSSA SSRKSSTSHP 180
KPRLPKVGKI GVCAMDAKVL SKPMRHILNR LIEHGEFETV IFGDKVILDE RIENWPTCDF 240
LISFFSSGFP LDKAIKYVKL RKPFIINDLI MQKILWDRRL CLQVLEAYNV PTPPRLEISR 300
DGGPRANEEL RAKLREHGVE VKPVEEPEWK MVDDDTLEVD GKTMTKPFVE KPVDGEDHNI 360
YIYYHSKNGG GGRRLFRKVG NKSSEFDPTL VHPRTEGSYI YEQFMDTDNF EDVKAYTIGE 420
NFCHAETRKS PVVDGIVRRN THGKEVRYIT ELSDEEKTIA GKVSKAFSQM ICGFDLLRVS 480
GKSYVIDVNG FSFVKDNKAY YDSCANILRS TFIEAKKKMD MEKKNLPIIR EEKEQKWVFK 540
GLAIIIRHAD RTPKQKFKHS FTSPIFISLL KGHKEEVVIR NVNDLKIVLQ ALRIALDEKA 600
GNPAKIKVLA NALEKKLNFP GTKIQLKPVL NKENEVEKVQ FILKWGGEPT HSAKYQATEL 660
GEQMRQDFDL LNKSILQNIK IFSSSERRVL HTAQYWTRAL FGADELGSDE ISIRKDLLDD 720
SNAAKDLMDK VKKKLKPLLR EGKEAPPQFA WPSKMPEPYL VIKRVVELMN YHKKIMDNNF 780
AKKDVNSMQT RWCTSEDPSL FKERWDKLFK EFNNAEKVDP SKISELYDTM KYDALHNRQF 840
LENIFDPGLP NEAIADELGS HSLVDRYPIN VLAKNNFKII DSHSMNNSGK NSSNSVGSLG 900
WVLESGKTST ARNPKSSSQF DEPRFMQLRE LYKLAKVLFD FICPKEYGIS DAEKLDIGLL 960
TSLPLAKQIL NDIGDMKNRE TPACVAYFTK ESHIYTLLNI IYESGIPMRI ARNALPELDY 1020
LSQITFELYE STDASGQKSH SIRLKMSPGC HTQDPLDVQL DDRHYISCIP KISLTKHLDM 1080
DYVQQKLRNK FTRVIMPPKF TPVNITSPNL SFQKRKTRRK SVSVEKLKRP ASSGSSSSTS 1140
VNKTLD 1146 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0003993; F:acid phosphatase activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0033857; F:diphosphoinositol-pentakisphosphate kinase activity; IEA:EC.
 GO:0000829; F:inositol heptakisphosphate kinase activity; IDA:SGD.
 GO:0052723; F:inositol hexakisphosphate 1-kinase activity; IDA:SGD.
 GO:0052724; F:inositol hexakisphosphate 3-kinase activity; IDA:SGD.
 GO:0000830; F:inositol hexakisphosphate 4-kinase activity; IDA:SGD.
 GO:0000832; F:inositol hexakisphosphate 5-kinase activity; IEA:EC.
 GO:0000831; F:inositol hexakisphosphate 6-kinase activity; IDA:SGD.
 GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
 GO:0032958; P:inositol phosphate biosynthetic process; IDA:SGD. 
Interpro
 IPR013651; ATP-grasp_RimK-type.
 IPR013816; ATP_grasp_subdomain_2.
 IPR000560; His_Pase_superF_clade-2. 
Pfam
 PF00328; His_Phos_2
 PF08443; RimK 
SMART
  
PROSITE
 PS00616; HIS_ACID_PHOSPHAT_1
 PS00778; HIS_ACID_PHOSPHAT_2 
PRINTS