CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023857
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transducin beta-like protein 2 
Protein Synonyms/Alias
 WS beta-transducin repeats protein; WS-betaTRP; Williams-Beuren syndrome chromosomal region 13 protein 
Gene Name
 TBL2 
Gene Synonyms/Alias
 WBSCR13; UNQ563/PRO1125 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
103MDFSSNGKYLATCADubiquitination[1]
120TIRIWSTKDFLQREHubiquitination[1, 2, 3, 4, 5]
263WEVCFGKKGEFQEVVubiquitination[1]
299RRMASVSKDGTWKLWubiquitination[1]
314DTDVEYKKKQDPYLLacetylation[6]
314DTDVEYKKKQDPYLLubiquitination[1]
315TDVEYKKKQDPYLLKacetylation[6]
315TDVEYKKKQDPYLLKubiquitination[1]
322KQDPYLLKTGRFEEAubiquitination[1, 3]
417EEMQGHLKRASNESTubiquitination[1]
440TQAQETLKSLGALKKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861
Functional Description
  
Sequence Annotation
 REPEAT 88 127 WD 1.
 REPEAT 134 174 WD 2.
 REPEAT 186 226 WD 3.
 REPEAT 228 267 WD 4.
 REPEAT 277 316 WD 5.
 REPEAT 329 367 WD 6.
 REPEAT 371 409 WD 7.
 MOD_RES 433 433 Phosphothreonine; by ATM or ATR.  
Keyword
 Complete proteome; Phosphoprotein; Polymorphism; Reference proteome; Repeat; WD repeat; Williams-Beuren syndrome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 447 AA 
Protein Sequence
MELSQMSELM GLSVLLGLLA LMATAAVARG WLRAGEERSG RPACQKANGF PPDKSSGSKK 60
QKQYQRIRKE KPQQHNFTHR LLAAALKSHS GNISCMDFSS NGKYLATCAD DRTIRIWSTK 120
DFLQREHRSM RANVELDHAT LVRFSPDCRA FIVWLANGDT LRVFKMTKRE DGGYTFTATP 180
EDFPKKHKAP VIDIGIANTG KFIMTASSDT TVLIWSLKGQ VLSTINTNQM NNTHAAVSPC 240
GRFVASCGFT PDVKVWEVCF GKKGEFQEVV RAFELKGHSA AVHSFAFSND SRRMASVSKD 300
GTWKLWDTDV EYKKKQDPYL LKTGRFEEAA GAAPCRLALS PNAQVLALAS GSSIHLYNTR 360
RGEKEECFER VHGECIANLS FDITGRFLAS CGDRAVRLFH NTPGHRAMVE EMQGHLKRAS 420
NESTRQRLQQ QLTQAQETLK SLGALKK 447 
Gene Ontology
  
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.