CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019937
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 281 
Protein Synonyms/Alias
  
Gene Name
 Znf281 
Gene Synonyms/Alias
 Zfp281 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
596EIKSCHDKSGIPDEVubiquitination[1]
619SGKSETQKEDPFNLTubiquitination[1]
658TQTPSNDKTSMLQEYubiquitination[1]
784AFQSSSQKLTSQKEQubiquitination[1]
838QAFGSQFKSGSRVPMubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs. Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes. 
Sequence Annotation
 ZN_FING 258 280 C2H2-type 1.
 ZN_FING 286 308 C2H2-type 2.
 ZN_FING 314 336 C2H2-type 3.
 ZN_FING 342 364 C2H2-type 4; atypical.
 MOD_RES 392 392 Phosphoserine (By similarity).
 MOD_RES 481 481 Phosphoserine (By similarity).
 MOD_RES 782 782 Phosphoserine (By similarity).
 MOD_RES 805 805 Phosphoserine (By similarity).  
Keyword
 Complete proteome; Differentiation; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 893 AA 
Protein Sequence
MKIGSGFLSG GGGPSSSGGS GSGGSSGSAS GGSGGGRRAE MEPTFPQSMV MFNHRLPPVT 60
SFTRPAGTAA PPPQCVLSSS TSAAPAAEPP PPPAPDMTFK KEPAASAAAF PSQRTSWGFL 120
QSLVSIKQEK PADPEEQPSH HHHHHHHYGG LFAGAEERSP GLGGGEGGSH GVIQDLSLLH 180
QHAQQQPAQH HRDVLLSSGS RTDEHGNQEP KQDANVKKAK RPKPESQGIK AKRKPSASSK 240
PLVGEGEGAV LSPSQKPHIC DHCSAAFRSS YHLRRHVLIH TGERPFQCSQ CSMGFIQKYL 300
LQRHEKIHSR EKPFGCDQCS MKFIQKYHME RHKRTHSGEK PYKCDTCQQY FSRTDRLLKH 360
RRTCGEAIAK GAASAEPGSS NHNSMGNLAV LSQGNTSSSR RKSKSKSIAI ENKEHKTGKT 420
NESQMSNNIN MQSYSVEMPT VSTSGSIIGT GIDELQKRVP KLIFKKGSRK NADKSYLNFV 480
SPLPDVVGQK SLSGKPGGSL GIVSNNSVET ISLLQSTSGK QGPISSNYDD AMQFSKKRRY 540
LPTASSNSAF SINVGHMVSQ QSVIQSAGVS VLDNEAPLSL IDSSALNAEI KSCHDKSGIP 600
DEVLQSILDQ YSGKSETQKE DPFNLTEPRV DLHTSGEHSE LVQEENLSPG TQTPSNDKTS 660
MLQEYSKYLQ QAFEKSTNAG FTLGHGFQFV SLSSPLHNHT LFPEKQIYTT SPLECGFGQS 720
VTSVLPSSLP KPPFGMLFGS QPGLYLSALD ATHQQLTPSQ ELDDLIDSQK NLETSSAFQS 780
SSQKLTSQKE QQKNLESSTS FQIPSQELAS QIDPQKDIEP RTTYQIENFA QAFGSQFKSG 840
SRVPMTFITN SNGEVDHRVR TSVSDFSGYT NMMSDVSEPC STRVKTPTSQ SYR 893 
Gene Ontology
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0001047; F:core promoter binding; IDA:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IMP:UniProtKB.
 GO:0003714; F:transcription corepressor activity; IMP:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0010172; P:embryonic body morphogenesis; IMP:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0048863; P:stem cell differentiation; IMP:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS