CPLM-009695

Genbank Nucleotide ID

Ubiquitin-conjugating enzyme E2 B

Protein Synonyms/Alias

E214K; RAD6 homolog B; HR6B; mHR6B; Ubiquitin carrier protein B; Ubiquitin-protein ligase B

Mus musculus (Mouse)

Position	Peptide	Type	References
66	FSEEYPNKPPTVRFL	acetylation	[1]

[1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]

Functional Description

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys- 164'. May be involved in neurite outgrowth (By similarity).

ACT_SITE 88 88 Glycyl thioester intermediate (By

ATP-binding; Cell membrane; Complete proteome; DNA damage; DNA repair; Ligase; Membrane; Nucleotide-binding; Nucleus; Reference proteome; Ubl conjugation pathway.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:Compara.
GO:0033503; C:HULC complex; ISS:UniProtKB.
GO:0000790; C:nuclear chromatin; IDA:MGI.
GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
GO:0005657; C:replication fork; IEA:Compara.
GO:0001741; C:XY body; IDA:MGI.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
GO:0060070; P:canonical Wnt receptor signaling pathway; IMP:UniProtKB.
GO:0032869; P:cellular response to insulin stimulus; IEA:Compara.
GO:0051026; P:chiasma assembly; IMP:MGI.
GO:0033522; P:histone H2A ubiquitination; IEA:Compara.
GO:0070076; P:histone lysine demethylation; IMP:MGI.
GO:0001701; P:in utero embryonic development; IGI:MGI.
GO:0006344; P:maintenance of chromatin silencing; IMP:MGI.
GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
GO:0043951; P:negative regulation of cAMP-mediated signaling; IEA:Compara.
GO:0033128; P:negative regulation of histone phosphorylation; IMP:MGI.
GO:0010845; P:positive regulation of reciprocal meiotic recombination; IMP:MGI.
GO:0006301; P:postreplication repair; IEA:Compara.
GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
GO:0051865; P:protein autoubiquitination; IEA:Compara.
GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
GO:0006513; P:protein monoubiquitination; IEA:Compara.
GO:0050821; P:protein stabilization; IEA:Compara.
GO:0042493; P:response to drug; IEA:Compara.
GO:0001666; P:response to hypoxia; IEA:Compara.
GO:0009411; P:response to UV; IEA:Compara.
GO:0007288; P:sperm axoneme assembly; IMP:MGI.
GO:0070193; P:synaptonemal complex organization; IMP:MGI.

IPR000608; UBQ-conjugat_E2.
IPR023313; UBQ-conjugating_AS.
IPR016135; UBQ-conjugating_enzyme/RWD.

PS00183; UBIQUITIN_CONJUGAT_1
PS50127; UBIQUITIN_CONJUGAT_2