UniProt Accession

 UBC12_MOUSE; P61082; O76069; Q8VC50 

Genbank Protein ID

 BC021792 

Genbank Nucleotide ID

 AAH21792.1 

Protein Name

 NEDD8-conjugating enzyme Ubc12 

Protein Synonyms/Alias

 NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M 

Gene Name

 Ube2m 

Gene Synonyms/Alias

 Ubc-rs2; Ubc12 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
3	*****MIKLFSLKQQ	acetylation	[1]
3	*****MIKLFSLKQQ	ubiquitination	[2]
8	MIKLFSLKQQKKEEE	acetylation	[1]
8	MIKLFSLKQQKKEEE	ubiquitination	[2]
36	AAQLRIQKDINELNL	ubiquitination	[2]
72	CPDEGFYKSGKFVFS	ubiquitination	[2]
75	EGFYKSGKFVFSFKV	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation (By similarity). 

Sequence Annotation

 REGION 1 26 Interaction with UBA3 (By similarity).
 REGION 27 57 Interaction with UBA3 (By similarity).
 ACT_SITE 111 111 Glycyl thioester intermediate (By
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 3 3 N6-acetyllysine (By similarity).
 MOD_RES 50 50 Phosphoserine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 183 AA 

Protein Sequence

Gene Ontology

 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0019788; F:NEDD8 ligase activity; ISS:UniProtKB.
 GO:0018169; F:ribosomal S6-glutamic acid ligase activity; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0043525; P:positive regulation of neuron apoptotic process; IEA:Compara.
 GO:0045116; P:protein neddylation; ISS:UniProtKB. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS