CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005375
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Exosome complex component RRP43 
Protein Synonyms/Alias
 Ribosomal RNA-processing protein 43 
Gene Name
 RRP43 
Gene Synonyms/Alias
 YCR035C; YCR35C; YCR522 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
71DAGNIDTKNNILGSNubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and cryptic unstable transcripts (CUTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and in RNA surveillance pathways, preventing translation of aberrant mRNAs. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. RRP43 is part of the hexameric ring of RNase PH domain-containing subunits proposed to form a central channel which threads RNA substrates for degradation. 
Sequence Annotation
 MOD_RES 26 26 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Exosome; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 394 AA 
Protein Sequence
MAESTTLETI EIHPITFPPE VLARISPELS LQRHLSLGIR PCLRKYEEFR DVAIENNTLS 60
RYADAGNIDT KNNILGSNVL KSGKTIVITS ITGGIIEETS AAIKDLDDFG EEELFEVTKE 120
EDIIANYASV YPVVEVERGR VGACTDEEMT ISQKLHDSIL HSRILPKKAL KVKAGVRSAN 180
EDGTFSVLYP DELEDDTLNE TNLKMKRKWS YVLYAKIVVL SRTGPVFDLC WNSLMYALQS 240
VKLPRAFIDE RASDLRMTIR TRGRSATIRE TYEIICDQTK SVPLMINAKN IAFASNYGIV 300
ELDPECQLQN SDNSEEEEVD IDMDKLNTVL IADLDTEAEE TSIHSTISIL AAPSGNYKQL 360
TLVGGGAKIT PEMIKRSLLL SRVRADDLST RFNI 394 
Gene Ontology
 GO:0000177; C:cytoplasmic exosome (RNase complex); IDA:SGD.
 GO:0000176; C:nuclear exosome (RNase complex); IDA:SGD.
 GO:0005730; C:nucleolus; IDA:SGD.
 GO:0005654; C:nucleoplasm; IDA:SGD.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0000467; P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0070651; P:nonfunctional rRNA decay; IC:SGD.
 GO:0071042; P:nuclear polyadenylation-dependent mRNA catabolic process; IMP:SGD.
 GO:0071035; P:nuclear polyadenylation-dependent rRNA catabolic process; IMP:SGD.
 GO:0071038; P:nuclear polyadenylation-dependent tRNA catabolic process; IDA:SGD.
 GO:0070478; P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay; IC:SGD.
 GO:0070481; P:nuclear-transcribed mRNA catabolic process, non-stop decay; IC:SGD.
 GO:0071051; P:polyadenylation-dependent snoRNA 3'-end processing; IC:SGD. 
Interpro
 IPR001247; ExoRNase_PH_dom1.
 IPR020568; Ribosomal_S5_D2-typ_fold. 
Pfam
 PF01138; RNase_PH 
SMART
  
PROSITE
  
PRINTS