UniProt Accession

 NLE1_HUMAN; Q9NVX2; O60868; Q59GJ8; Q9BU54 

Genbank Protein ID

 AK001320; AB209111; AC022916; BC002884; BC012075; AJ005257 

Genbank Nucleotide ID

 BAA91621.1; BAD92348.1; AAH02884.2; AAH12075.1; CAA06444.1 

Protein Name

 Notchless protein homolog 1 

Protein Synonyms/Alias

  

Gene Name

 NLE1 

Gene Synonyms/Alias

 HUSSY-07 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
176	RKLASGCKNGQILLW	ubiquitination	[1]
189	LWDPSTGKQVGRTLA	ubiquitination	[1]
224	RYVASSSKDGSVRIW	ubiquitination	[1, 2]
326	QGSLQELKERALSRY	ubiquitination	[1, 2, 3]
439	GSSDSTLKVWDVKAQ	ubiquitination	[1]
447	VWDVKAQKLAMDLPG	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

  

Sequence Annotation

 REPEAT 112 151 WD 1.
 REPEAT 154 193 WD 2.
 REPEAT 197 241 WD 3.
 REPEAT 244 282 WD 4.
 REPEAT 325 366 WD 5.
 REPEAT 370 409 WD 6.
 REPEAT 412 451 WD 7.
 REPEAT 454 484 WD 8.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 79 79 Phosphoserine.  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 485 AA 

Protein Sequence

Gene Ontology

 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0001826; P:inner cell mass cell differentiation; IEA:Compara.
 GO:0007219; P:Notch signaling pathway; IEA:Compara. 

Interpro

 IPR020472; G-protein_beta_WD-40_rep.
 IPR001632; Gprotein_B.
 IPR012972; NLE.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF08154; NLE
 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS

 PR00319; GPROTEINB.
 PR00320; GPROTEINBRPT.