UniProt Accession

 KPYK1_ECOLI; P0AD61; P14178; P76921; P78165; P78231 

Genbank Protein ID

 M24636; U68703; U00096; AP009048 

Genbank Nucleotide ID

 AAA24392.1; AAB47952.1; AAC74746.1; BAA15445.2 

Protein Name

 Pyruvate kinase I 

Protein Synonyms/Alias

 PK-1 

Gene Name

 pykF 

Gene Synonyms/Alias

 b1676; JW1666 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
13	IVCTIGPKTESEEML	acetylation	[1, 2]
22	ESEEMLAKMLDAGMN	acetylation	[2]
56	NLRNVMSKTGKTAAI	acetylation	[1]
59	NVMSKTGKTAAILLD	acetylation	[2]
68	AAILLDTKGPEIRTM	acetylation	[1, 2]
76	GPEIRTMKLEGGNDV	acetylation	[1, 2, 3]
145	EGNKVICKVLNNGDL	acetylation	[1, 2]
156	NGDLGENKGVNLPGV	acetylation	[2]
173	ALPALAEKDKQDLIF	acetylation	[2]
208	IEIREHLKAHGGENI	acetylation	[1]
266	AQKMMIEKCIRARKV	acetylation	[2]
272	EKCIRARKVVITATQ	acetylation	[1]
286	QMLDSMIKNPRPTRA	acetylation	[1, 2]
317	MLSGESAKGKYPLEA	acetylation	[1, 2]
319	SGESAKGKYPLEAVS	acetylation	[1, 2, 3]
368	GAVETAEKLDAPLIV	acetylation	[1, 2]
382	VVATQGGKSARAVRK	acetylation	[1, 2]
389	KSARAVRKYFPDATI	acetylation	[1, 2]
404	LALTTNEKTAHQLVL	acetylation	[2]
413	AHQLVLSKGVVPQLV	acetylation	[2]
421	GVVPQLVKEITSTDD	acetylation	[1, 2]
434	DDFYRLGKELALQSG	acetylation	[2]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 

Functional Description

  

Sequence Annotation

 METAL 34 34 Potassium (By similarity).
 METAL 36 36 Potassium (By similarity).
 METAL 66 66 Potassium (By similarity).
 METAL 67 67 Potassium; via carbonyl oxygen (By
 METAL 222 222 Magnesium (By similarity).
 METAL 246 246 Magnesium (By similarity).
 BINDING 32 32 Substrate (By similarity).
 BINDING 245 245 Substrate; via amide nitrogen (By
 BINDING 246 246 Substrate; via amide nitrogen (By
 BINDING 278 278 Substrate (By similarity).
 MOD_RES 76 76 N6-acetyllysine.
 MOD_RES 319 319 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Allosteric enzyme; ATP-binding; Complete proteome; Direct protein sequencing; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Potassium; Pyruvate; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 470 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0030955; F:potassium ion binding; IEA:InterPro.
 GO:0004743; F:pyruvate kinase activity; IEA:EC.
 GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway. 

Interpro

 IPR001697; Pyr_Knase.
 IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
 IPR011037; Pyrv_Knase-like_insert_dom.
 IPR015794; Pyrv_Knase_a/b.
 IPR018209; Pyrv_Knase_AS.
 IPR015793; Pyrv_Knase_brl.
 IPR015795; Pyrv_Knase_C.
 IPR015806; Pyrv_Knase_insert_dom. 

Pfam

 PF00224; PK
 PF02887; PK_C 

SMART

  

PROSITE

 PS00110; PYRUVATE_KINASE 

PRINTS

 PR01050; PYRUVTKNASE.