CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016221
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable E3 ubiquitin-protein ligase DTX2 
Protein Synonyms/Alias
 Protein deltex-2; Deltex2; hDTX2; RING finger protein 58 
Gene Name
 DTX2 
Gene Synonyms/Alias
 KIAA1528; RNF58 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
249QAFAPYNKPSLSGARacetylation[1]
249QAFAPYNKPSLSGARubiquitination[2, 3, 4]
479CKTIYGEKTGTQPQGubiquitination[3]
553RKVLELLKVAWKRRLacetylation[5]
553RKVLELLKVAWKRRLubiquitination[2, 3, 4]
557ELLKVAWKRRLIFTVacetylation[5]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786
Functional Description
 Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity. 
Sequence Annotation
 DOMAIN 8 97 WWE 1.
 DOMAIN 98 174 WWE 2.
 ZN_FING 412 473 RING-type.
 MOD_RES 249 249 N6-acetyllysine.
 MOD_RES 360 360 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Ligase; Metal-binding; Notch signaling pathway; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 622 AA 
Protein Sequence
MAMAPSPSLV QVYTSPAAVA VWEWQDGLGT WHPYSATVCS FIEQQFVQQK GQRFGLGSLA 60
HSIPLGQADP SLAPYIIDLP SWTQFRQDTG TMRAVRRHLF PQHSAPGRGV VWEWLSDDGS 120
WTAYEASVCD YLEQQVARGN QLVDLAPLGY NYTVNYTTHT QTNKTSSFCR SVRRQAGPPY 180
PVTTIIAPPG HTGVACSCHQ CLSGSRTGPV SGRYRHSMTN LPAYPVPQHP PHRTASVFGT 240
HQAFAPYNKP SLSGARSAPR LNTTNAWGAA PPSLGSQPLY RSSLSHLGPQ HLPPGSSTSG 300
AVSASLPSGP SSSPGSVPAT VPMQMPKPSR VQQALAGMTS VLMSAIGLPV CLSRAPQPTS 360
PPASRLASKS HGSVKRLRKM SVKGATPKPE PEPEQVIKNY TEELKVPPDE DCIICMEKLS 420
TASGYSDVTD SKAIGSLAVG HLTKCSHAFH LLCLLAMYCN GNKDGSLQCP SCKTIYGEKT 480
GTQPQGKMEV LRFQMSLPGH EDCGTILIVY SIPHGIQGPE HPNPGKPFTA RGFPRQCYLP 540
DNAQGRKVLE LLKVAWKRRL IFTVGTSSTT GETDTVVWNE IHHKTEMDRN ITGHGYPDPN 600
YLQNVLAELA AQGVTEDCLE QQ 622 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway. 
Interpro
 IPR004170; WWE-dom.
 IPR018123; WWE-dom_subgr.
 IPR018957; Znf_C3HC4_RING-type.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF02825; WWE
 PF00097; zf-C3HC4 
SMART
 SM00184; RING
 SM00678; WWE 
PROSITE
 PS50918; WWE
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS