CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008864
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 S-adenosylmethionine synthase 
Protein Synonyms/Alias
 AdoMet synthase; MAT; Methionine adenosyltransferase 
Gene Name
 metK 
Gene Synonyms/Alias
 metE; BSU30550 
Created Date
 July 27, 2013 
Organism
 Bacillus subtilis (strain 168) 
NCBI Taxa ID
 224308 
Lysine Modification
Position
Peptide
Type
References
191VEYDENNKPVRIDAIacetylation[1]
Reference
 [1] The acetylproteome of Gram-positive model bacterium Bacillus subtilis.
 Kim D, Yu BJ, Kim JA, Lee YJ, Choi SG, Kang S, Pan JG.
 Proteomics. 2013 May;13(10-11):1726-36. [PMID: 23468065] 
Functional Description
 Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme (By similarity). 
Sequence Annotation
 NP_BIND 274 281 ATP (Potential).
 METAL 19 19 Magnesium (By similarity).
 METAL 45 45 Potassium (By similarity).
 METAL 278 278 Potassium (By similarity).
 METAL 286 286 Magnesium (By similarity).  
Keyword
 ATP-binding; Cobalt; Complete proteome; Cytoplasm; Magnesium; Metal-binding; Nucleotide-binding; One-carbon metabolism; Potassium; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 400 AA 
Protein Sequence
MSKNRRLFTS ESVTEGHPDK ICDQISDSIL DEILKKDPNA RVACETSVTT GLVLVSGEIT 60
TSTYVDIPKT VRQTIKEIGY TRAKYGFDAE TCAVLTSIDE QSADIAMGVD QALEAREGTM 120
SDEEIEAIGA GDQGLMFGYA CNETKELMPL PISLAHKLAR RLSEVRKEDI LPYLRPDGKT 180
QVTVEYDENN KPVRIDAIVI STQHHPEITL EQIQRNIKEH VINPVVPEEL IDEETKYFIN 240
PTGRFVIGGP QGDAGLTGRK IIVDTYGGYA RHGGGAFSGK DATKVDRSAA YAARYVAKNI 300
VAAELADSCE VQLAYAIGVA QPVSISINTF GSGKASEEKL IEVVRNNFDL RPAGIIKMLD 360
LRRPIYKQTA AYGHFGRHDV DLPWERTDKA EQLRKEALGE 400 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0004478; F:methionine adenosyltransferase activity; IEA:HAMAP.
 GO:0006730; P:one-carbon metabolic process; IEA:HAMAP.
 GO:0006556; P:S-adenosylmethionine biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR022631; ADOMET_SYNTHASE_CS.
 IPR022630; S-AdoMet_synt_C.
 IPR022629; S-AdoMet_synt_central.
 IPR022628; S-AdoMet_synt_N.
 IPR002133; S-AdoMet_synthetase.
 IPR022636; S-AdoMet_synthetase_sfam. 
Pfam
 PF02773; S-AdoMet_synt_C
 PF02772; S-AdoMet_synt_M
 PF00438; S-AdoMet_synt_N 
SMART
  
PROSITE
 PS00376; ADOMET_SYNTHASE_1
 PS00377; ADOMET_SYNTHASE_2 
PRINTS