UniProt Accession

 DHB5_MOUSE; P70694 

Genbank Protein ID

 D45850; AF110414; AF110408; AF110409; AF110410; AF110411; AF110412; AF110413; BC056643 

Genbank Nucleotide ID

 BAA08285.1; AAD41250.1; AAD41250.1; AAD41250.1; AAD41250.1; AAD41250.1; AAD41250.1; AAD41250.1; AAH56643.1 

Protein Name

 Estradiol 17 beta-dehydrogenase 5 

Protein Synonyms/Alias

 17-beta-HSD 5 

Gene Name

 Akr1c6 

Gene Synonyms/Alias

 Hsd17b5 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
4	****MDSKQQTVRLS	acetylation	[1]
4	****MDSKQQTVRLS	succinylation	[1]
4	****MDSKQQTVRLS	ubiquitination	[2]
31	YAPQEVPKSKATEAT	ubiquitination	[2]
39	SKATEATKIAIDAGF	ubiquitination	[2]
59	ASMYQNEKEVGLAIR	ubiquitination	[2]
75	KIADGTVKREDIFYT	ubiquitination	[2]
123	IHFPMAMKPGENYLP	ubiquitination	[2]
131	PGENYLPKDENGKLI	ubiquitination	[2]
155	WEAMEKCKDAGLAKS	ubiquitination	[2]
161	CKDAGLAKSIGVSNF	ubiquitination	[2]
178	RQLEKILKKPGLKYK	acetylation	[3]
178	RQLEKILKKPGLKYK	ubiquitination	[2]
185	KKPGLKYKPVCNQVE	acetylation	[3]
185	KKPGLKYKPVCNQVE	ubiquitination	[2]
201	HPYLNQGKLLDFCRS	acetylation	[3]
201	HPYLNQGKLLDFCRS	ubiquitination	[2]
209	LLDFCRSKDIVLVAY	acetylation	[3]
209	LLDFCRSKDIVLVAY	ubiquitination	[2]
225	ALGSHREKQWVDQSS	ubiquitination	[2]
246	PVLGSMAKKYNRTPA	ubiquitination	[2]
270	RGVVVLAKSFSEKRI	ubiquitination	[2]
301	KVLDDLNKNIRYISG	acetylation	[3, 4, 5, 6]
301	KVLDDLNKNIRYISG	ubiquitination	[2]
312	YISGSSFKDHPDFPF	ubiquitination	[2]

Reference

 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [5] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Active toward androgens, estrogens, and xenobiotic substrates. Also exhibits low 20 alpha-HSD activity. Shows a- stereospecificity in hydrogen transfer between cofactors and substrates (A-specific). Preferentially catalyzes the reduction of 4-androstenedione, 5-alpha-androstane-3,17-dione, androsterone and dehydroepiandrosterone to testosterone, dihydrotestosterone, 5- alpha-androstane-3-alpha,17-beta-diol and 5-androstene-3-beta,17- beta-diol, respectively. 

Sequence Annotation

 NP_BIND 13 22 NADP (Potential).
 NP_BIND 217 280 NADP (By similarity).
 ACT_SITE 55 55 Proton donor (By similarity).
 BINDING 117 117 Substrate (By similarity).
 MOD_RES 310 310 Phosphoserine.  

Keyword

 Complete proteome; Direct protein sequencing; Lipid biosynthesis; Lipid metabolism; NADP; Oxidoreductase; Phosphoprotein; Reference proteome; Steroid biosynthesis. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 323 AA 

Protein Sequence

Gene Ontology

 GO:0004033; F:aldo-keto reductase (NADP) activity; IDA:MGI.
 GO:0004303; F:estradiol 17-beta-dehydrogenase activity; IDA:MGI.
 GO:0006694; P:steroid biosynthetic process; IDA:MGI. 

Interpro

 IPR001395; Aldo/ket_red.
 IPR018170; Aldo/ket_reductase_CS.
 IPR020471; Aldo/keto_reductase_subgr.
 IPR023210; NADP_OxRdtase_dom. 

Pfam

 PF00248; Aldo_ket_red 

SMART

  

PROSITE

 PS00798; ALDOKETO_REDUCTASE_1
 PS00062; ALDOKETO_REDUCTASE_2
 PS00063; ALDOKETO_REDUCTASE_3 

PRINTS

 PR00069; ALDKETRDTASE.