CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030507
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitotic spindle assembly checkpoint protein MAD1 
Protein Synonyms/Alias
 cDNA FLJ33640 fis, clone BRAMY2023482, highly similar to Mitotic spindle assembly checkpoint protein MAD1 
Gene Name
 MAD1L1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
221ENERLLAKLQSWERLubiquitination[1]
389EMELKMLKSQSSSAEubiquitination[1]
412EADTLRLKVEELEGEubiquitination[1, 2]
507AASLPSSKEVAELKKubiquitination[2]
522QVESAELKNQRLKEVubiquitination[1, 2]
533LKEVFQTKIQEFRKAubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 626 AA 
Protein Sequence
MLPARGCVRK RTVWPRLARV LIVTLLTLEL SYAPLPCQLS GVPYNTGDPV GRWARPCIWP 60
CPWHTTINAL KGRISELQWS VMDQEMRVKR LESEKQELQE QLDLQHKKCQ EANQKIQELQ 120
ASQEARADHE QQIKDLEQKL SLQEQDAAIV KNMKSELVRL PRLERELKQL REESAHLREM 180
RETNGLLQEE LEGLQRKLGR QEKMQETLVG LELENERLLA KLQSWERLDQ TMGLSIRTPE 240
DLSRFVVELQ QRELALKDKN SAVTSSARGL EKARQQLQEE LRQVSGQLLE ERKKRETHEA 300
LARRLQKRVL LLTKERDGMR AILGSYDSEL TPAEYSPQLT RRMREAEDMV QKVHSHSAEM 360
EAQLSQALEE LGGQKQRADM LEMELKMLKS QSSSAEQSFL FSREEADTLR LKVEELEGER 420
SRLEEEKRML EAQLERRALQ GDYDQSRTKV LHMSLNPTSV ARQRLREDHS QLQAECERLR 480
GLLRAMERGG TVPADLEAAA ASLPSSKEVA ELKKQVESAE LKNQRLKEVF QTKIQEFRKA 540
CYTLTGYQID ITTENQYRLT SLYAEHPGDC LIFKATSPSG SKMQLLETEF SHTVGELIEV 600
HLRRQDSIPA FLSSLTLELF SRQTVA 626 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IDA:HPA.
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0007094; P:mitotic spindle assembly checkpoint; IEA:InterPro. 
Interpro
 IPR008672; Mad1. 
Pfam
 PF05557; MAD 
SMART
  
PROSITE
  
PRINTS