CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002441
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Fatty acid synthase subunit beta 
Protein Synonyms/Alias
 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; Enoyl-[acyl-carrier-protein] reductase [NADH]; [Acyl-carrier-protein] acetyltransferase; [Acyl-carrier-protein] malonyltransferase; S-acyl fatty acid synthase thioesterase 
Gene Name
 FAS1 
Gene Synonyms/Alias
 YKL182W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
124VKTKELIKNYITARIacetylation[1]
364NSHLPAGKQVEISLVubiquitination[2]
445LINKDLVKNNVSFNAubiquitination[2]
453NNVSFNAKDIQIPVYacetylation[1]
489CIIRLPVKWETTTQFacetylation[1]
557VTSNGLKKNPNWLEEacetylation[1]
568WLEEYHPKLIKNKSGacetylation[1]
582GKIFVETKFSKLIGRacetylation[1]
706GLKYLGLKPGSIDAIubiquitination[2]
756PMLQMYSKIRRHPNIacetylation[1]
756PMLQMYSKIRRHPNIubiquitination[2]
809GSRVMIAKEVKTSPDacetylation[1]
831CTGVPDDKWEQTYKKubiquitination[2]
838KWEQTYKKPTGGIVTubiquitination[2]
856EMGEPIHKIATRGVMacetylation[1]
877ETIFNLPKNKLVPTLacetylation[1]
877ETIFNLPKNKLVPTLubiquitination[2]
879IFNLPKNKLVPTLEAacetylation[1]
879IFNLPKNKLVPTLEAubiquitination[2]
887LVPTLEAKRDYIISRacetylation[1]
887LVPTLEAKRDYIISRubiquitination[2]
901RLNADFQKPWFATVNubiquitination[2]
975QSYSLLDKPDEAIEKacetylation[1]
1083GIHDGHIKKLLHQYYacetylation[1]
1192SNGNTSSKTVVTLSEubiquitination[2]
1206EPVQGELKPTVILKLacetylation[1]
1215TVILKLLKENIIQMEacetylation[1]
1215TVILKLLKENIIQMEubiquitination[2]
1336VGWRAIIKAIFPNTVacetylation[1]
1364YKMIPGAKPLQVGDVubiquitination[2, 3]
1436PVYQMHIKTSKDIAVacetylation[1]
1439QMHIKTSKDIAVLRSacetylation[1]
1484ANIFSSVKCFGPIKVacetylation[1]
1639INGRKLIKFETRNEDacetylation[1]
1685MDLYKTSKAAQDVWNubiquitination[2]
1753LKTEKIFKEINEHSTacetylation[1]
1768SYTFRSEKGLLSATQacetylation[1]
1768SYTFRSEKGLLSATQubiquitination[2]
1786PALTLMEKAAFEDLKubiquitination[2]
1918TNVLNFIKLQKIDIIacetylation[1]
1921LNFIKLQKIDIIELQacetylation[1]
1998FLKKNIIKENVKVARacetylation[1]
2002NIIKENVKVARLAGKubiquitination[2]
2009KVARLAGKYIPNLTAacetylation[1]
2017YIPNLTAKPFQVTKEacetylation[1]
2017YIPNLTAKPFQVTKEubiquitination[2]
2047EIIDNWEKYEQS***acetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Fatty acid synthetase catalyzes the formation of long- chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The beta subunit contains domains for: [acyl-carrier-protein] acetyltransferase and malonyltransferase, S-acyl fatty acid synthase thioesterase, enoyl-[acyl-carrier-protein] reductase, and 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase. 
Sequence Annotation
 DOMAIN 1523 1648 MaoC-like.
 REGION 1 468 Acetyltransferase.
 REGION 480 868 Enoyl reductase.
 REGION 1144 1626 Dehydratase.
 REGION 1627 1845 Malonyl/palmitoyl transferase.
 ACT_SITE 274 274 For acetyltransferase activity (By
 ACT_SITE 1808 1808 For malonyltransferase activity (By
 MOD_RES 733 733 Phosphothreonine.
 MOD_RES 1121 1121 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Fatty acid biosynthesis; Fatty acid metabolism; Hydrolase; Lipid biosynthesis; Lipid metabolism; Lyase; Multifunctional enzyme; NAD; NADP; Oxidoreductase; Phosphoprotein; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2051 AA 
Protein Sequence
MDAYSTRPLT LSHGSLEHVL LVPTASFFIA SQLQEQFNKI LPEPTEGFAA DDEPTTPAEL 60
VGKFLGYVSS LVEPSKVGQF DQVLNLCLTE FENCYLEGND IHALAAKLLQ ENDTTLVKTK 120
ELIKNYITAR IMAKRPFDKK SNSALFRAVG EGNAQLVAIF GGQGNTDDYF EELRDLYQTY 180
HVLVGDLIKF SAETLSELIR TTLDAEKVFT QGLNILEWLE NPSNTPDKDY LLSIPISCPL 240
IGVIQLAHYV VTAKLLGFTP GELRSYLKGA TGHSQGLVTA VAIAETDSWE SFFVSVRKAI 300
TVLFFIGVRC YEAYPNTSLP PSILEDSLEN NEGVPSPMLS ISNLTQEQVQ DYVNKTNSHL 360
PAGKQVEISL VNGAKNLVVS GPPQSLYGLN LTLRKAKAPS GLDQSRIPFS ERKLKFSNRF 420
LPVASPFHSH LLVPASDLIN KDLVKNNVSF NAKDIQIPVY DTFDGSDLRV LSGSISERIV 480
DCIIRLPVKW ETTTQFKATH ILDFGPGGAS GLGVLTHRNK DGTGVRVIVA GTLDINPDDD 540
YGFKQEIFDV TSNGLKKNPN WLEEYHPKLI KNKSGKIFVE TKFSKLIGRP PLLVPGMTPC 600
TVSPDFVAAT TNAGYTIELA GGGYFSAAGM TAAIDSVVSQ IEKGSTFGIN LIYVNPFMLQ 660
WGIPLIKELR SKGYPIQFLT IGAGVPSLEV ASEYIETLGL KYLGLKPGSI DAISQVINIA 720
KAHPNFPIAL QWTGGRGGGH HSFEDAHTPM LQMYSKIRRH PNIMLIFGSG FGSADDTYPY 780
LTGEWSTKFD YPPMPFDGFL FGSRVMIAKE VKTSPDAKKC IAACTGVPDD KWEQTYKKPT 840
GGIVTVRSEM GEPIHKIATR GVMLWKEFDE TIFNLPKNKL VPTLEAKRDY IISRLNADFQ 900
KPWFATVNGQ ARDLATMTYE EVAKRLVELM FIRSTNSWFD VTWRTFTGDF LRRVEERFTK 960
SKTLSLIQSY SLLDKPDEAI EKVFNAYPAA REQFLNAQDI DHFLSMCQNP MQKPVPFVPV 1020
LDRRFEIFFK KDSLWQSEHL EAVVDQDVQR TCILHGPVAA QFTKVIDEPI KSIMDGIHDG 1080
HIKKLLHQYY GDDESKIPAV EYFGGESPVD VQSQVDSSSV SEDSAVFKAT SSTDEESWFK 1140
ALAGSEINWR HASFLCSFIT QDKMFVSNPI RKVFKPSQGM VVEISNGNTS SKTVVTLSEP 1200
VQGELKPTVI LKLLKENIIQ MEMIENRTMD GKPVSLPLLY NFNPDNGFAP ISEVMEDRNQ 1260
RIKEMYWKLW IDEPFNLDFD PRDVIKGKDF EITAKEVYDF THAVGNNCED FVSRPDRTML 1320
APMDFAIVVG WRAIIKAIFP NTVDGDLLKL VHLSNGYKMI PGAKPLQVGD VVSTTAVIES 1380
VVNQPTGKIV DVVGTLSRNG KPVMEVTSSF FYRGNYTDFE NTFQKTVEPV YQMHIKTSKD 1440
IAVLRSKEWF QLDDEDFDLL NKTLTFETET EVTFKNANIF SSVKCFGPIK VELPTKETVE 1500
IGIVDYEAGA SHGNPVVDFL KRNGSTLEQK VNLENPIPIA VLDSYTPSTN EPYARVSGDL 1560
NPIHVSRHFA SYANLPGTIT HGMFSSASVR ALIENWAADS VSSRVRGYTC QFVDMVLPNT 1620
ALKTSIQHVG MINGRKLIKF ETRNEDDVVV LTGEAEIEQP VTTFVFTGQG SQEQGMGMDL 1680
YKTSKAAQDV WNRADNHFKD TYGFSILDIV INNPVNLTIH FGGEKGKRIR ENYSAMIFET 1740
IVDGKLKTEK IFKEINEHST SYTFRSEKGL LSATQFTQPA LTLMEKAAFE DLKSKGLIPA 1800
DATFAGHSLG EYAALASLAD VMSIESLVEV VFYRGMTMQV AVPRDELGRS NYGMIAINPG 1860
RVAASFSQEA LQYVVERVGK RTGWLVEIVN YNVENQQYVA AGDLRALDTV TNVLNFIKLQ 1920
KIDIIELQKS LSLEEVEGHL FEIIDEASKK SAVKPRPLKL ERGFACIPLV GISVPFHSTY 1980
LMNGVKPFKS FLKKNIIKEN VKVARLAGKY IPNLTAKPFQ VTKEYFQDVY DLTGSEPIKE 2040
IIDNWEKYEQ S 2051 
Gene Ontology
 GO:0005835; C:fatty acid synthase complex; IDA:SGD.
 GO:0005811; C:lipid particle; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0019171; F:3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity; IDA:SGD.
 GO:0047451; F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; IEA:EC.
 GO:0004313; F:[acyl-carrier-protein] S-acetyltransferase activity; IDA:SGD.
 GO:0004314; F:[acyl-carrier-protein] S-malonyltransferase activity; IDA:SGD.
 GO:0004318; F:enoyl-[acyl-carrier-protein] reductase (NADH) activity; IEA:EC.
 GO:0004319; F:enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; IDA:SGD.
 GO:0004321; F:fatty-acyl-CoA synthase activity; IEA:EC.
 GO:0016295; F:myristoyl-[acyl-carrier-protein] hydrolase activity; IEA:EC.
 GO:0004320; F:oleoyl-[acyl-carrier-protein] hydrolase activity; IEA:EC.
 GO:0016296; F:palmitoyl-[acyl-carrier-protein] hydrolase activity; IEA:EC.
 GO:0016409; F:palmitoyltransferase activity; IDA:SGD.
 GO:0006633; P:fatty acid biosynthetic process; IMP:SGD. 
Interpro
 IPR001227; Ac_transferase_dom.
 IPR014043; Acyl_transferase.
 IPR016035; Acyl_Trfase/lysoPLipase.
 IPR013565; DUF1729.
 IPR003965; Fatty_acid_synthase.
 IPR016452; Fatty_acid_Synthase_bsu_fun.
 IPR002539; MaoC_dom. 
Pfam
 PF00698; Acyl_transf_1
 PF08354; DUF1729
 PF01575; MaoC_dehydratas 
SMART
  
PROSITE
  
PRINTS
 PR01483; FASYNTHASE.