CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015891
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Elongation factor Tu GTP-binding domain-containing protein 1 
Protein Synonyms/Alias
 Elongation factor-like 1; Protein FAM42A 
Gene Name
 EFTUD1 
Gene Synonyms/Alias
 EFL1; FAM42A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
14DKMIQLQKNTANIRNubiquitination[1]
401ALKAAFMKCGSEDTAubiquitination[1]
528KIFVLGPKYSPLEFLacetylation[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits. 
Sequence Annotation
 NP_BIND 26 33 GTP (By similarity).
 NP_BIND 92 96 GTP (By similarity).
 NP_BIND 146 149 GTP (By similarity).
 MOD_RES 528 528 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Elongation factor; GTP-binding; Nucleotide-binding; Polymorphism; Protein biosynthesis; Reference proteome; Ribosome biogenesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1120 AA 
Protein Sequence
MVLNSLDKMI QLQKNTANIR NICVLAHVDH GKTTLADCLI SSNGIISSRL AGKLRYMDSR 60
EDEQIRGITM KSSAISLHYA TGNEEYLINL IDSPGHVDFS SEVSTAVRIC DGCIIVVDAV 120
EGVCPQTQAV LRQAWLENIR PVLVINKIDR LIVELKFTPQ EAYSHLKNIL EQINALTGTL 180
FTSKVLEERA ERETESQVNP NSEQGEQVYD WSTGLEDTDD SHLYFSPEQG NVVFTSAIDG 240
WGFGIEHFAR IYSQKIGIKK EVLMKTLWGD YYINMKAKKI MKGDQAKGKK PLFVQLILEN 300
IWSLYDAVLK KDKDKIDKIV TSLGLKIGAR EARHSDPKVQ INAICSQWLP ISHAVLAMVC 360
QKLPSPLDIT AERVERLMCT GSQTFDSFPP ETQALKAAFM KCGSEDTAPV IIFVSKMFAV 420
DAKALPQNKP RPLTQEEIAQ RRERARQRHA EKLAAAQGQA PLEPTQDGSA IETCPKGEEP 480
RGDEQQVESM TPKPVLQEEN NQESFIAFAR VFSGVARRGK KIFVLGPKYS PLEFLRRVPL 540
GFSAPPDGLP QVPHMAYCAL ENLYLLMGRE LEYLEEVPPG NVLGIGGLQD FVLKSATLCS 600
LPSCPPFIPL NFEATPIVRV AVEPKHPSEM PQLVKGMKLL NQADPCVQIL IQETGEHVLV 660
TAGEVHLQRC LDDLKERFAK IHISVSEPII PFRETITKPP KVDMVNEEIG KQQKVAVIHQ 720
MKEDQSKIPE GIQVDSDGLI TITTPNKLAT LSVRAMPLPE EVTQILEENS DLIRSMEQLT 780
SSLNEGENTH MIHQKTQEKI WEFKGKLEQH LTGRRWRNIV DQIWSFGPRK CGPNILVNKS 840
EDFQNSVWTG PADKASKEAS RYRDLGNSIV SGFQLATLSG PMCEEPLMGV CFVLEKWDLS 900
KFEEQGASDL AKEGQEENET CSGGNENQEL QDGCSEAFEK RTSQKGESPL TDCYGPFSGQ 960
LIATMKEACR YALQVKPQRL MAAMYTCDIM ATGDVLGRVY AVLSKREGRV LQEEMKEGTD 1020
MFIIKAVLPV AESFGFADEI RKRTSGLASP QLVFSHWEII PSDPFWVPTT EEEYLHFGEK 1080
ADSENQARKY MNAVRKRKGL YVEEKIVEHA EKQRTLSKNK 1120 
Gene Ontology
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IDA:UniProtKB.
 GO:0043022; F:ribosome binding; IMP:UniProtKB.
 GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
 GO:0042256; P:mature ribosome assembly; IMP:UniProtKB. 
Interpro
 IPR000795; EF_GTP-bd_dom.
 IPR009022; EFG_III-V.
 IPR000640; EFG_V.
 IPR027417; P-loop_NTPase.
 IPR020568; Ribosomal_S5_D2-typ_fold.
 IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
 IPR005225; Small_GTP-bd_dom.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF00679; EFG_C
 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2 
SMART
 SM00838; EFG_C 
PROSITE
 PS00301; EFACTOR_GTP 
PRINTS
 PR00315; ELONGATNFCT.