CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036114
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Eukaryotic translation initiation factor 4E 
Protein Synonyms/Alias
  
Gene Name
 EIF4E 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11KQNKKERKKMRAEDGmethylation[1]
12QNKKERKKMRAEDGEmethylation[1]
64ANPEHYIKHPLQNRWubiquitination[2]
82FFKNDKSKTWQANLRubiquitination[3]
147RWLITLNKQQRRSDLacetylation[4]
147RWLITLNKQQRRSDLubiquitination[3, 5, 6]
187AVVNVRAKGDKIAIWacetylation[7]
190NVRAKGDKIAIWTTEubiquitination[3]
234SHADTATKSGSTTKNubiquitination[3]
Reference
 [1] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Initiation factor; Protein biosynthesis; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 245 AA 
Protein Sequence
MKKKQNKKER KKMRAEDGEN DAIKKQAESL RESQETTPTP NPPTTEEEKT ESNQEVANPE 60
HYIKHPLQNR WALWFFKNDK SKTWQANLRL ISKFDTVEDF WALYNHIQLS SNLMPGCDYS 120
LFKDGIEPMW EDEKNKRGGR WLITLNKQQR RSDLDRFWLE TLLCLIGESF DDYSDDVCGA 180
VVNVRAKGDK IAIWTTECEN REAVTHIGRV YKERLGLPPK IVIGYQSHAD TATKSGSTTK 240
NRFVV 245 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW. 
Interpro
 IPR023398; TIF_eIF4e-like_dom.
 IPR001040; TIF_eIF_4E.
 IPR019770; TIF_eIF_4E_CS. 
Pfam
 PF01652; IF4E 
SMART
  
PROSITE
 PS00813; IF4E 
PRINTS