CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013513
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bromodomain testis-specific protein 
Protein Synonyms/Alias
 Cancer/testis antigen 9; CT9; RING3-like protein 
Gene Name
 BRDT 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
349QEYKDAYKFAADVRLubiquitination[1, 2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin. 
Sequence Annotation
 DOMAIN 44 116 Bromo 1.
 DOMAIN 287 359 Bromo 2.
 DOMAIN 500 582 NET.
 MOTIF 209 220 Nuclear localization signal.
 BINDING 109 109 Histone H4K5ac (By similarity).
 BINDING 109 109 JQ1 inhibitor.
 BINDING 114 114 Histone H4K5ac (By similarity).  
Keyword
 3D-structure; Activator; Alternative splicing; Bromodomain; Chromatin regulator; Coiled coil; Complete proteome; Differentiation; Meiosis; mRNA processing; mRNA splicing; Nucleus; Polymorphism; Reference proteome; Repeat; Spermatogenesis; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 947 AA 
Protein Sequence
MTCTCRQDSK QLRMSLPSRQ TAIIVNPPPP EYINTKKNGR LTNQLQYLQK VVLKDLWKHS 60
FSWPFQRPVD AVKLQLPDYY TIIKNPMDLN TIKKRLENKY YAKASECIED FNTMFSNCYL 120
YNKPGDDIVL MAQALEKLFM QKLSQMPQEE QVVGVKERIK KGTQQNIAVS SAKEKSSPSA 180
TEKVFKQQEI PSVFPKTSIS PLNVVQGASV NSSSQTAAQV TKGVKRKADT TTPATSAVKA 240
SSEFSPTFTE KSVALPPIKE NMPKNVLPDS QQQYNVVKTV KVTEQLRHCS EILKEMLAKK 300
HFSYAWPFYN PVDVNALGLH NYYDVVKNPM DLGTIKEKMD NQEYKDAYKF AADVRLMFMN 360
CYKYNPPDHE VVTMARMLQD VFETHFSKIP IEPVESMPLC YIKTDITETT GRENTNEASS 420
EGNSSDDSED ERVKRLAKLQ EQLKAVHQQL QVLSQVPFRK LNKKKEKSKK EKKKEKVNNS 480
NENPRKMCEQ MRLKEKSKRN QPKKRKQQFI GLKSEDEDNA KPMNYDEKRQ LSLNINKLPG 540
DKLGRVVHII QSREPSLSNS NPDEIEIDFE TLKASTLREL EKYVSACLRK RPLKPPAKKI 600
MMSKEELHSQ KKQELEKRLL DVNNQLNSRK RQTKSDKTQP SKAVENVSRL SESSSSSSSS 660
SESESSSSDL SSSDSSDSES EMFPKFTEVK PNDSPSKENV KKMKNECIPP EGRTGVTQIG 720
YCVQDTTSAN TTLVHQTTPS HVMPPNHHQL AFNYQELEHL QTVKNISPLQ ILPPSGDSEQ 780
LSNGITVMHP SGDSDTTMLE SECQAPVQKD IKIKNADSWK SLGKPVKPSG VMKSSDELFN 840
QFRKAAIEKE VKARTQELIR KHLEQNTKEL KASQENQRDL GNGLTVESFS NKIQNKCSGE 900
EQKEHQQSSE AQDKSKLWLL KDRDLARQKE QERRRREAMV GTIDMTLQSD IMTMFENNFD 960 
Gene Ontology
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0070577; F:histone acetyl-lysine binding; ISS:UniProtKB.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0006338; P:chromatin remodeling; ISS:UniProtKB.
 GO:0001207; P:histone displacement; ISS:UniProtKB.
 GO:0007141; P:male meiosis I; ISS:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0051039; P:positive regulation of transcription during meiosis; ISS:UniProtKB.
 GO:0043484; P:regulation of RNA splicing; ISS:UniProtKB.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
 GO:0007283; P:spermatogenesis; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR001487; Bromodomain.
 IPR018359; Bromodomain_CS.
 IPR027353; NET_dom. 
Pfam
 PF00439; Bromodomain 
SMART
 SM00297; BROMO 
PROSITE
 PS00633; BROMODOMAIN_1
 PS50014; BROMODOMAIN_2
 PS51525; NET 
PRINTS
 PR00503; BROMODOMAIN.