CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003316
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosome-binding ATPase YchF 
Protein Synonyms/Alias
  
Gene Name
 ychF 
Gene Synonyms/Alias
 engD; gtp1; b1203; JW1194 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
59DQLAEIVKPQRTLPTacetylation[1]
156KKAKGGDKDAKAELAacetylation[1]
159KGGDKDAKAELAVLEacetylation[1]
188LDLSAEEKAAIRYLSacetylation[1]
288TYFTAGVKEVRAWTIacetylation[1]
307TAPQAAGKIHTDFEKacetylation[1]
314KIHTDFEKGFIRAQTacetylation[1]
331FEDFITYKGEQGAKEacetylation[1]
347GKMRAEGKDYIVKDGacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner. Does not hydrolyze GTP. 
Sequence Annotation
 DOMAIN 4 109 G.
 NP_BIND 12 17 ATP (By similarity).  
Keyword
 ATP-binding; Complete proteome; Direct protein sequencing; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 363 AA 
Protein Sequence
MGFKCGIVGL PNVGKSTLFN ALTKAGIEAA NFPFCTIEPN TGVVPMPDPR LDQLAEIVKP 60
QRTLPTTMEF VDIAGLVKGA SKGEGLGNQF LTNIRETEAI GHVVRCFEND NIIHVSGKVN 120
PADDIEVINT ELALADLDTC ERAIHRVQKK AKGGDKDAKA ELAVLEKCLP QLENAGMLRA 180
LDLSAEEKAA IRYLSFLTLK PTMYIANVNE DGFENNPYLD QVREIAAKEG SVVVPVCAAV 240
EADIAELDDE ERDEFMQELG LEEPGLNRVI RAGYKLLNLQ TYFTAGVKEV RAWTIPVGAT 300
APQAAGKIHT DFEKGFIRAQ TISFEDFITY KGEQGAKEAG KMRAEGKDYI VKDGDVMNFL 360
FNV 363 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IDA:EcoCyc.
 GO:0004857; F:enzyme inhibitor activity; IMP:EcoCyc.
 GO:0005525; F:GTP binding; IEA:InterPro.
 GO:0043023; F:ribosomal large subunit binding; IDA:EcoCyc.
 GO:0043022; F:ribosome binding; IDA:EcoCyc.
 GO:0006979; P:response to oxidative stress; IEP:EcoCyc. 
Interpro
 IPR012675; Beta-grasp_dom.
 IPR004396; CHP00092.
 IPR013029; DUF933.
 IPR006073; GTP_binding_domain.
 IPR027417; P-loop_NTPase.
 IPR012676; TGS-like.
 IPR023192; TGS-like_dom. 
Pfam
 PF01926; MMR_HSR1
 PF06071; YchF-GTPase_C 
SMART
  
PROSITE
  
PRINTS
 PR00326; GTP1OBG.