CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022436
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Maspardin 
Protein Synonyms/Alias
 Acid cluster protein 33; Spastic paraplegia 21 autosomal recessive Mast syndrome protein; Spastic paraplegia 21 protein 
Gene Name
 SPG21 
Gene Synonyms/Alias
 ACP33; BM-019; GL010 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***MGEIKVSPDYNWubiquitination[1, 2]
21RGTVPLKKIIVDDDDubiquitination[2]
30IVDDDDSKIWSLYDAubiquitination[2, 3]
124KFAEYTHKSPRVHSLubiquitination[2, 4]
213NSYVEPHKIRDIPVTubiquitination[2]
251NARRAHLKTGGNFPYubiquitination[4]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 May play a role as a negative regulatory factor in CD4- dependent T-cell activation. 
Sequence Annotation
 MOD_RES 304 304 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Endosome; Golgi apparatus; Hereditary spastic paraplegia; Membrane; Neurodegeneration; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 308 AA 
Protein Sequence
MGEIKVSPDY NWFRGTVPLK KIIVDDDDSK IWSLYDAGPR SIRCPLIFLP PVSGTADVFF 60
RQILALTGWG YRVIALQYPV YWDHLEFCDG FRKLLDHLQL DKVHLFGASL GGFLAQKFAE 120
YTHKSPRVHS LILCNSFSDT SIFNQTWTAN SFWLMPAFML KKIVLGNFSS GPVDPMMADA 180
IDFMVDRLES LGQSELASRL TLNCQNSYVE PHKIRDIPVT IMDVFDQSAL STEAKEEMYK 240
LYPNARRAHL KTGGNFPYLC RSAEVNLYVQ IHLLQFHGTK YAAIDPSMVS AEELEVQKGS 300
LGISQEEQ 308 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0030140; C:trans-Golgi network transport vesicle; IDA:UniProtKB.
 GO:0050851; P:antigen receptor-mediated signaling pathway; IC:UniProtKB.
 GO:0008219; P:cell death; IEA:UniProtKB-KW. 
Interpro
 IPR026151; Maspardin. 
Pfam
  
SMART
  
PROSITE
  
PRINTS