UniProt Accession

 API5_HUMAN; Q9BZZ5; B4DGR0; B4DRJ2; D3DR21; O15441; Q9Y4J7 

Genbank Protein ID

 U83857; Y15906; AF229253; AF229254; AY311389; BT007093; AY265973; AK294724; AK299288; AC087276; CH471064; CH471064; BC017709; W40304 

Genbank Nucleotide ID

 AAB86528.1; CAA75867.1; AAK00737.1; AAK00738.1; AAQ76714.1; AAP35756.1; AAO89077.1; BAG57871.1; BAG61304.1; EAW68098.1; EAW68099.1; AAH17709.1 

Protein Name

 Apoptosis inhibitor 5 

Protein Synonyms/Alias

 API-5; Antiapoptosis clone 11 protein; AAC-11; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; FIF; Protein XAGL 

Gene Name

 API5 

Gene Synonyms/Alias

 MIG8 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
36	QVILDGVKGGTKEKR	ubiquitination	[1]
51	LAAQFIPKFFKHFPE	ubiquitination	[1]
54	QFIPKFFKHFPELAD	ubiquitination	[2]
84	SIRRQAIKELPQFAT	ubiquitination	[1]
153	IVRERAIKFLSTKLK	ubiquitination	[2]
251	QAVPLFSKNVHSTRF	acetylation	[3]
487	IYNPPSGKYSSNLGN	ubiquitination	[1]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. 

Sequence Annotation

 REGION 2 360 ARM-like and Heat-like helical repeats.
 REGION 370 391 Leucine-zipper.
 MOTIF 454 475 Nuclear localization signal.
 MOD_RES 251 251 N6-acetyllysine.
 MOD_RES 462 462 Phosphoserine.
 MOD_RES 464 464 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Apoptosis; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 524 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; NAS:UniProtKB.
 GO:0005681; C:spliceosomal complex; ISS:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB. 

Interpro

 IPR008383; API5.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold. 

Pfam

 PF05918; API5 

SMART

  

PROSITE

  

PRINTS