CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000061
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sulfhydryl oxidase 1 
Protein Synonyms/Alias
 hQSOX; Quiescin Q6 
Gene Name
 QSOX1 
Gene Synonyms/Alias
 QSCN6; UNQ2520/PRO6013 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
194YLALIFEKGGSYLGRubiquitination[1]
285VAPTTANKIAPTVWKubiquitination[2]
676RRVGRSSKQLVDIPEubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. In fibroblasts, it may have tumor- suppressing capabilities being involved in growth regulation. 
Sequence Annotation
 DOMAIN 36 156 Thioredoxin.
 DOMAIN 396 503 ERV/ALR sulfhydryl oxidase.
 NP_BIND 478 485 FAD.
 ACT_SITE 70 70 Nucleophile (Probable).
 ACT_SITE 73 73 Nucleophile (Probable).
 BINDING 401 401 FAD.
 BINDING 408 408 FAD.
 BINDING 412 412 FAD.
 BINDING 451 451 FAD.
 BINDING 455 455 FAD.
 BINDING 500 500 FAD.
 BINDING 503 503 FAD.
 CARBOHYD 130 130 N-linked (GlcNAc...).
 CARBOHYD 243 243 N-linked (GlcNAc...) (Potential).
 CARBOHYD 575 575 N-linked (GlcNAc...) (Potential).
 DISULFID 70 73 Redox-active (Probable).
 DISULFID 393 405
 DISULFID 449 452
 DISULFID 509 512  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Disulfide bond; FAD; Flavoprotein; Glycoprotein; Golgi apparatus; Membrane; Oxidoreductase; Polymorphism; Reference proteome; Secreted; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 747 AA 
Protein Sequence
MRRCNSGSGP PPSLLLLLLW LLAVPGANAA PRSALYSPSD PLTLLQADTV RGAVLGSRSA 60
WAVEFFASWC GHCIAFAPTW KALAEDVKAW RPALYLAALD CAEETNSAVC RDFNIPGFPT 120
VRFFKAFTKN GSGAVFPVAG ADVQTLRERL IDALESHHDT WPPACPPLEP AKLEEIDGFF 180
ARNNEEYLAL IFEKGGSYLG REVALDLSQH KGVAVRRVLN TEANVVRKFG VTDFPSCYLL 240
FRNGSVSRVP VLMESRSFYT AYLQRLSGLT REAAQTTVAP TTANKIAPTV WKLADRSKIY 300
MADLESALHY ILRIEVGRFP VLEGQRLVAL KKFVAVLAKY FPGRPLVQNF LHSVNEWLKR 360
QKRNKIPYSF FKTALDDRKE GAVLAKKVNW IGCQGSEPHF RGFPCSLWVL FHFLTVQAAR 420
QNVDHSQEAA KAKEVLPAIR GYVHYFFGCR DCASHFEQMA AASMHRVGSP NAAVLWLWSS 480
HNRVNARLAG APSEDPQFPK VQWPPRELCS ACHNERLDVP VWDVEATLNF LKAHFSPSNI 540
ILDFPAAGSA ARRDVQNVAA APELAMGALE LESRNSTLDP GKPEMMKSPT NTTPHVPAEG 600
PEASRPPKLH PGLRAAPGQE PPEHMAELQR NEQEQPLGQW HLSKRDTGAA LLAESRAEKN 660
RLWGPLEVRR VGRSSKQLVD IPEGQLEARA GRGRGQWLQV LGGGFSYLDI SLCVGLYSLS 720
FMGLLAMYTY FQAKIRALKG HAGHPAA 747 
Gene Ontology
 GO:0005615; C:extracellular space; IDA:UniProtKB.
 GO:0030173; C:integral to Golgi membrane; IDA:UniProtKB.
 GO:0016971; F:flavin-linked sulfhydryl oxidase activity; IDA:UniProtKB.
 GO:0003756; F:protein disulfide isomerase activity; IDA:UniProtKB.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro. 
Interpro
 IPR017905; ERV/ALR_sulphydryl_oxidase.
 IPR006863; Evr1_Alr.
 IPR012336; Thioredoxin-like_fold.
 IPR013766; Thioredoxin_domain. 
Pfam
 PF04777; Evr1_Alr
 PF00085; Thioredoxin 
SMART
  
PROSITE
 PS51324; ERV_ALR
 PS51352; THIOREDOXIN_2 
PRINTS