UniProt Accession

 CHSTC_HUMAN; Q9NRB3; A4D1Z9; Q502W3; Q9NXY7 

Genbank Protein ID

 AF239822; AJ289131; AY358574; AK313484; CH236953; BC002918; BC095492 

Genbank Nucleotide ID

 AAF81692.1; CAB92133.1; AAQ88937.1; BAG36268.1; EAL23955.1; AAH02918.1; AAH95492.1 

Protein Name

 Carbohydrate sulfotransferase 12 

Protein Synonyms/Alias

 Chondroitin 4-O-sulfotransferase 2; Chondroitin 4-sulfotransferase 2; C4ST-2; C4ST2; Sulfotransferase Hlo 

Gene Name

 CHST12 

Gene Synonyms/Alias

 UNQ500/PRO1017 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
86	KQSDLPRKETEQPPA	ubiquitination	[1]
142	SSLAFPTKERAFDDI	ubiquitination	[1, 2]
172	AIYCYVPKVACTNWK	ubiquitination	[1]
240	VKLKKYTKFLFVRDP	ubiquitination	[1]
258	LISAFRSKFELENEE	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor. 

Sequence Annotation

 NP_BIND 171 177 PAPS (By similarity).
 NP_BIND 245 253 PAPS (By similarity).
 CARBOHYD 134 134 N-linked (GlcNAc...) (Potential).
 CARBOHYD 209 209 N-linked (GlcNAc...) (Potential).
 CARBOHYD 280 280 N-linked (GlcNAc...) (Potential).
 CARBOHYD 370 370 N-linked (GlcNAc...) (Potential).  

Keyword

 Carbohydrate metabolism; Complete proteome; Glycoprotein; Golgi apparatus; Membrane; Polymorphism; Reference proteome; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 414 AA 

Protein Sequence

Gene Ontology

 GO:0030173; C:integral to Golgi membrane; NAS:UniProtKB.
 GO:0050656; F:3'-phosphoadenosine 5'-phosphosulfate binding; NAS:UniProtKB.
 GO:0047756; F:chondroitin 4-sulfotransferase activity; IDA:UniProtKB.
 GO:0016051; P:carbohydrate biosynthetic process; IEA:InterPro.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0030206; P:chondroitin sulfate biosynthetic process; IDA:UniProtKB.
 GO:0030208; P:dermatan sulfate biosynthetic process; IDA:UniProtKB. 

Interpro

 IPR018011; Carb_sulfotransferase-rel.
 IPR005331; Sulfotransferase. 

Pfam

 PF03567; Sulfotransfer_2 

SMART

  

PROSITE

  

PRINTS