CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020945
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NEDD4-like E3 ubiquitin-protein ligase WWP2 
Protein Synonyms/Alias
 WW domain-containing protein 2 
Gene Name
 Wwp2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
828FCIDRVGKETWLPRSubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; regulates POU5F1 protein level during differentiation of embryonal carcinoma cells (ECCs) but not in undifferentiated ECCs and embryonic stem cells (ESCs). Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation. 
Sequence Annotation
 DOMAIN 20 100 C2.
 DOMAIN 300 333 WW 1.
 DOMAIN 330 363 WW 2.
 DOMAIN 405 437 WW 3.
 DOMAIN 444 477 WW 4.
 DOMAIN 536 870 HECT.
 ACT_SITE 838 838 Glycyl thioester intermediate (By
 MOD_RES 211 211 Phosphoserine (By similarity).  
Keyword
 Complete proteome; Ligase; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 870 AA 
Protein Sequence
MASASSSRAG VALPFEKSQL TLKVVSAKPK VHNRQPRINS YVEVAVDGLP SETKKTGKRI 60
GSSELLWNEI IVLNVTAQSH LDLKVWSCHT LRNELLGTAS VNLSNVLKNN GGKMENTQLT 120
LNLQTENKGS VVSGGELTIF LDGPTVDLGS VPNGSAVTDG SQPPSRESSG TAIAPETRHQ 180
PPSTNCFGGR SRTHRHSGGS ARTATAASEQ SPGARNRHRQ PVKNSSSSGL ANGTVNEEPT 240
PASEPEESSV VGVTSLPAAA LSVSSNPNTT SLPAQSTPAE GEEASTSGTQ QLPAAAQAPD 300
ALPAGWEQRE LPNGRVYYVD HNTKTTTWER PLPPGWEKRT DPRGRFYYVD HNTRTTTWQR 360
PTAEYVRNYE QWQSQRNQLQ GAMQHFSQRF LYQSSSASTD HDPLGPLPPG WEKRQDNGRV 420
YYVNHNTRTT QWEDPRTQGM IQEPALPPGW EMKYTSEGVR YFVDHNTRTT TFKDPRPGFE 480
SGTKQGSPGA YDRSFRWKYH QFRFLCHSNA LPSHVKISVS RQTLFEDSFQ QIMNMKPYDL 540
RRRLYIIMRG EEGLDYGGIA REWFFLLSHE VLNPMYCLFE YAGKNNYCLQ INPASSINPD 600
HLTYFRFIGR FIAMALYHGK FIDTGFTLPF YKRMLNKRPT LKDLESIDPE FYNSIVWIKE 660
NNLEECGLEL FFIQDMEILG KVTTHELKEG GENIRVTEEN KEEYIMLLTD WRFTRGVEEQ 720
TKAFLDGFNE VAPLEWLRYF DEKELELMLC GMQEIDMSDW QKNAIYRHYT KSSKQIQWFW 780
QVVKEMDNEK RIRLLQFVTG TCRLPVGGFA ELIGSNGPQK FCIDRVGKET WLPRSHTCFN 840
RLDLPPYKSY EQLKEKLLYA IEETEGFGQE 870 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:BHF-UCL.
 GO:0005634; C:nucleus; IDA:BHF-UCL.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
 GO:0051224; P:negative regulation of protein transport; IEA:Compara.
 GO:0043433; P:negative regulation of sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
 GO:0032410; P:negative regulation of transporter activity; IEA:Compara.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IMP:UniProtKB.
 GO:0070534; P:protein K63-linked ubiquitination; IMP:UniProtKB.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IBA:RefGenome.
 GO:0042391; P:regulation of membrane potential; IEA:Compara.
 GO:1901016; P:regulation of potassium ion transmembrane transporter activity; IEA:Compara. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR024928; E3_ub_ligase_SMURF1.
 IPR000569; HECT.
 IPR001202; WW_dom. 
Pfam
 PF00632; HECT
 PF00397; WW 
SMART
 SM00239; C2
 SM00119; HECTc
 SM00456; WW 
PROSITE
 PS50004; C2
 PS50237; HECT
 PS01159; WW_DOMAIN_1
 PS50020; WW_DOMAIN_2 
PRINTS