CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031940
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase RNF14 
Protein Synonyms/Alias
 cDNA FLJ58057, highly similar to RING finger protein 14 
Gene Name
 RNF14 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
175YHGVSPCKVTAEKLMubiquitination[1]
180PCKVTAEKLMDLRNEubiquitination[1]
196LQADEANKRLLDQRYubiquitination[1, 2, 3]
210YGKRVIQKALEEMESubiquitination[1, 2, 4]
218ALEEMESKEWLEKNSubiquitination[1]
223ESKEWLEKNSKSCPCubiquitination[1]
226EWLEKNSKSCPCCGTubiquitination[1]
268LSRANPYKHFNDPGSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Metal-binding; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 298 AA 
Protein Sequence
MSSEDREAQE DELLALASIY DGDEFRKAES VQGGETRIYL DLPQNFKIFV SGNSNECLQN 60
SGFEYTICFL PPLVLNFELP PDYPSSSPPS FTLSGKWLSP TQVKELVEAE LFARYDRLLL 120
QSSLDLMADV VYCPRPCCQL PVMQEPGCTM GICSSCNFAF CTLCRLTYHG VSPCKVTAEK 180
LMDLRNEYLQ ADEANKRLLD QRYGKRVIQK ALEEMESKEW LEKNSKSCPC CGTPIEKLDG 240
CNKMTCTGCM QYFCWICMGS LSRANPYKHF NDPGSPCFNR LFYAVDVDDD IWEDEVED 298 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0008270; F:zinc ion binding; IEA:InterPro. 
Interpro
 IPR006575; RWD-domain.
 IPR016135; UBQ-conjugating_enzyme/RWD.
 IPR002867; Znf_C6HC.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF01485; IBR
 PF05773; RWD 
SMART
 SM00647; IBR
 SM00591; RWD 
PROSITE
 PS50908; RWD 
PRINTS