UniProt Accession

 MYLK2_HUMAN; Q9H1R3; Q569L1; Q96I84 

Genbank Protein ID

 AF325549; AJ272502; AL160175; BC007753; BC069627; BC092413; BC127622 

Genbank Nucleotide ID

 AAK15494.1; CAC81354.1; CAC10006.1; AAH07753.1; AAH69627.1; AAH92413.1; AAI27623.1 

Protein Name

 Myosin light chain kinase 2, skeletal/cardiac muscle 

Protein Synonyms/Alias

 MLCK2 

Gene Name

 MYLK2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
561	LKSQILLKKYLMKRR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N- terminus of a myosin light chain. 

Sequence Annotation

 DOMAIN 285 540 Protein kinase.
 NP_BIND 291 299 ATP (By similarity).
 REGION 574 586 Calmodulin-binding (By similarity).
 ACT_SITE 406 406 Proton acceptor (By similarity).
 BINDING 314 314 ATP (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).  

Keyword

 3D-structure; Acetylation; ATP-binding; Calmodulin-binding; Cardiomyopathy; Complete proteome; Cytoplasm; Disease mutation; Kinase; Nucleotide-binding; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 596 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0030017; C:sarcomere; IC:BHF-UCL.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005516; F:calmodulin binding; ISS:BHF-UCL.
 GO:0004683; F:calmodulin-dependent protein kinase activity; ISS:BHF-UCL.
 GO:0004687; F:myosin light chain kinase activity; IDA:BHF-UCL.
 GO:0055007; P:cardiac muscle cell differentiation; IEA:Compara.
 GO:0060048; P:cardiac muscle contraction; IC:BHF-UCL.
 GO:0055008; P:cardiac muscle tissue morphogenesis; IMP:BHF-UCL.
 GO:0007274; P:neuromuscular synaptic transmission; IEA:Compara.
 GO:0018107; P:peptidyl-threonine phosphorylation; IDA:UniProtKB.
 GO:0010628; P:positive regulation of gene expression; IDA:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB.
 GO:0032971; P:regulation of muscle filament sliding; IDA:BHF-UCL.
 GO:0014816; P:satellite cell differentiation; IEA:Compara.
 GO:0035914; P:skeletal muscle cell differentiation; IDA:UniProtKB. 

Interpro

 IPR020636; Ca/CaM-dep_Ca-dep_prot_Kinase.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS