CPLM-009354

Genbank Nucleotide ID

Pterin-4-alpha-carbinolamine dehydratase

Protein Synonyms/Alias

PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD

Homo sapiens (Human)

Position	Peptide	Type	References
41	IFKQFHFKDFNRAFG	acetylation	[1]
41	IFKQFHFKDFNRAFG	ubiquitination	[2, 3, 4]
59	RVALQAEKLDHHPEW	ubiquitination	[2, 4]
72	EWFNVYNKVHITLST	ubiquitination	[5]

[1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcription. Regulates the dimerization of homeodomain protein HNF1A and enhances its transcriptional activity.

REGION 61 63 Substrate binding (By similarity).
REGION 78 81 Substrate binding (By similarity).
MOD_RES 2 2 N-acetylalanine.

Acetylation; Activator; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Lyase; Nucleus; Reference proteome; Tetrahydrobiopterin biosynthesis; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0005634; C:nucleus; IDA:HPA.
GO:0008124; F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity; EXP:Reactome.
GO:0004505; F:phenylalanine 4-monooxygenase activity; IEA:Compara.
GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
GO:0034641; P:cellular nitrogen compound metabolic process; TAS:Reactome.
GO:0006559; P:L-phenylalanine catabolic process; TAS:Reactome.
GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
GO:0051291; P:protein heterooligomerization; IEA:Compara.
GO:0051289; P:protein homotetramerization; IEA:Compara.
GO:0043496; P:regulation of protein homodimerization activity; IEA:Compara.
GO:0006729; P:tetrahydrobiopterin biosynthetic process; IEA:UniProtKB-KW.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR001533; Trans/pterin_deHydtase.