CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-027152
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Citrate synthase 
Protein Synonyms/Alias
  
Gene Name
 Csl 
Gene Synonyms/Alias
 mCG_19869 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
76YGGMRGMKGLVYETSacetylation[1, 2, 3, 4, 5, 6]
76YGGMRGMKGLVYETSsuccinylation[5]
76YGGMRGMKGLVYETSubiquitination[7]
215KLPCVAAKIYRNLYRacetylation[2, 4, 5]
215KLPCVAAKIYRNLYRsuccinylation[5]
215KLPCVAAKIYRNLYRubiquitination[7]
366CQREFALKHLPKDPMacetylation[2, 3, 6]
370FALKHLPKDPMFKLVacetylation[1, 3, 4, 5, 6]
370FALKHLPKDPMFKLVsuccinylation[5]
370FALKHLPKDPMFKLVubiquitination[7]
450GFPLERPKSMSTDALacetylation[8]
Reference
 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [7] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [8] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405
Functional Description
  
Sequence Annotation
  
Keyword
 3D-structure; Complete proteome; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 466 AA 
Protein Sequence
MALLTAAAWF LGTKNPPCLV LAARHASASS TNLKDVLRNL IPKEQARIKT FRKKHGKTVV 60
GQITVDMMYG GMRGMKGLVY ETSVLDPDEG IRFRGYSIPE CQKLLPKAKG GKEPLPEGLF 120
WLLVTGQMPT EEQVSWLSQE WVKRAALPSH VVTMLDNFPT KLHPMSQLSA AITVLNNESN 180
FARAYAQGMN RTKYWELTYE DCMDLLAKLP CVAAKIYRNL YREDRNIEAI DSKLDWSHNF 240
TNMLGYTDPQ FTELMRLYLT IHSDHEGGNV SAHTSHLVGS ALSDPYLSFA AALNGLAGPL 300
HGLANQEVLV WLTQLQKEVG EDASDEKLKN YIWNTLNSGR VVPGYGHAVL RKTDPRYSCQ 360
REFALKHLPK DPMFKLVGQL YKIVPDILLE QGKAKNPWPN VDAHSGVLLQ YYGMREMNYY 420
TVLFGVSRAL GVLSQLIWSR ALGFPLERPK SMSTDALMKF VNSESG 466 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro. 
Interpro
 IPR016142; Citrate_synth-like_lrg_a-sub.
 IPR002020; Citrate_synthase-like.
 IPR016141; Citrate_synthase-like_core.
 IPR019810; Citrate_synthase_AS.
 IPR010109; Citrate_synthase_euk. 
Pfam
 PF00285; Citrate_synt 
SMART
  
PROSITE
 PS00480; CITRATE_SYNTHASE 
PRINTS
 PR00143; CITRTSNTHASE.