CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007202
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic initiation factor 4F subunit p150 
Protein Synonyms/Alias
 eIF-4F p150; eIF4F p150; eIF4G1; mRNA cap-binding protein complex subunit p150 
Gene Name
 TIF4631 
Gene Synonyms/Alias
 YGR162W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
21KQESAALKQTGDDQQubiquitination[1]
153LKEQHKAKLQSQERSacetylation[2]
169VSPQPESKLKETSDSubiquitination[1]
171PQPESKLKETSDSTSubiquitination[1]
273KSAEPEVKQETPAEEubiquitination[1]
689TDETNEGKTGPKLVLacetylation[3]
689TDETNEGKTGPKLVLubiquitination[1]
839ISSRIKFKLIDIKELacetylation[3]
844KFKLIDIKELRHDKNacetylation[3]
906RDAPPASKDSFITTRubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Component of the eIF4F complex, which interacts with the mRNA cap structure and serves as an initial point of assembly for the translation apparatus. Stimulates translation by interaction with polyadenylate-binding protein PAB1, bringing the 5'- and 3'- ends of the mRNA in proximity. The formation of this circular mRNP structure appears to be critical for the synergistic effects of the cap and the poly(A) tail in facilitating translation initiation, recycling of ribosomes, and mRNA stability. TIF4631 is probably essential when TIF4632 is missing. 
Sequence Annotation
 DOMAIN 607 850 MIF4G.
 REGION 188 299 Interaction with PAB1.
 MOD_RES 181 181 Phosphothreonine.
 MOD_RES 888 888 Phosphothreonine.
 MOD_RES 908 908 Phosphoserine.
 MOD_RES 948 948 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 952 AA 
Protein Sequence
MTDETAHPTQ SASKQESAAL KQTGDDQQES QQQRGYTNYN NGSNYTQKKP YNSNRPHQQR 60
GGKFGPNRYN NRGNYNGGGS FRGGHMGANS SNVPWTGYYN NYPVYYQPQQ MAAAGSAPAN 120
PIPVEEKSPV PTKIEITTKS GEHLDLKEQH KAKLQSQERS TVSPQPESKL KETSDSTSTS 180
TPTPTPSTND SKASSEENIS EAEKTRRNFI EQVKLRKAAL EKKRKEQLEG SSGNNNIPMK 240
TTPENVEEKG SDKPEVTEKT KPAEEKSAEP EVKQETPAEE GEQGEKGQIK EESTPKVLTF 300
AERLKLKKQQ KEREEKTEGK ENKEVPVQEE TKSAIESAPV PPSEQVKEET EVAETEQSNI 360
DESATTPAIP TKSDEAEAEV EAEAGDAGTK IGLEAEIETT TDETDDGTNT VSHILNVLKD 420
ATPIEDVFSF NYPEGIEGPD IKYKKEHVKY TYGPTFLLQF KDKLNVKADA EWVQSTASKI 480
VIPPGMGRGN RSRDSGRFGN NSSRGHDFRN TSVRNMDDRA NSRTSSKRRS KRMNDDRRSN 540
RSYTSRRDRE RGSYRNEEKR EDDKPKEEVA PLVPSANRWV PKFKSKKTEK KLAPDGKTEL 600
LDKDEVERKM KSLLNKLTLE MFDAISSEIL AIANISVWET NGETLKAVIE QIFLKACDEP 660
HWSSMYAQLC GKVVKELNPD ITDETNEGKT GPKLVLHYLV ARCHAEFDKG WTDKLPTNED 720
GTPLEPEMMS EEYYAAASAK RRGLGLVRFI GFLYRLNLLT GKMMFECFRR LMKDLTDSPS 780
EETLESVVEL LNTVGEQFET DSFRTGQATL EGSQLLDSLF GILDNIIQTA KISSRIKFKL 840
IDIKELRHDK NWNSDKKDNG PKTIQQIHEE EERQRQLKNN SRSNSRRTNN SSNRHSFRRD 900
APPASKDSFI TTRTYSQRNS QRAPPPKEEP AAPTSTATNM FSALMGESDD EE 952 
Gene Ontology
 GO:0010494; C:cytoplasmic stress granule; IDA:SGD.
 GO:0016281; C:eukaryotic translation initiation factor 4F complex; IMP:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005840; C:ribosome; TAS:SGD.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; TAS:SGD.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0042273; P:ribosomal large subunit biogenesis; IMP:SGD.
 GO:0016070; P:RNA metabolic process; IEA:InterPro.
 GO:0034063; P:stress granule assembly; IMP:SGD. 
Interpro
 IPR016024; ARM-type_fold.
 IPR022745; eIF4G1_eIF4E-bd.
 IPR016021; MIF4-like_typ_1/2/3.
 IPR003890; MIF4G-like_typ-3. 
Pfam
 PF12152; eIF_4G1
 PF02854; MIF4G 
SMART
 SM00543; MIF4G 
PROSITE
  
PRINTS