CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004359
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphoribosylamine--glycine ligase 
Protein Synonyms/Alias
 GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase 
Gene Name
 purD 
Gene Synonyms/Alias
 b4005; JW3969 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
78GPEAPLVKGVVDTFRacetylation[1]
90TFRAAGLKIFGPTAGacetylation[1]
105AAQLEGSKAFTKDFLacetylation[1]
109EGSKAFTKDFLARHKacetylation[1]
116KDFLARHKIPTAEYQacetylation[1]
221DHKRVGDKDTGPNTGacetylation[1]
283IDKQGNPKVIEFNCRacetylation[1]
322CESKLDEKTSEWDERacetylation[1]
370KVFHAGTKLADDEQVacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
  
Sequence Annotation
 DOMAIN 109 316 ATP-grasp.
 NP_BIND 135 196 ATP (By similarity).
 METAL 286 286 Magnesium or manganese (By similarity).
 METAL 288 288 Magnesium or manganese (By similarity).  
Keyword
 3D-structure; ATP-binding; Complete proteome; Ligase; Magnesium; Manganese; Metal-binding; Nucleotide-binding; Purine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 429 AA 
Protein Sequence
MKVLVIGNGG REHALAWKAA QSPLVETVFV APGNAGTALE PALQNVAIGV TDIPALLDFA 60
QNEKIDLTIV GPEAPLVKGV VDTFRAAGLK IFGPTAGAAQ LEGSKAFTKD FLARHKIPTA 120
EYQNFTEVEP ALAYLREKGA PIVIKADGLA AGKGVIVAMT LEEAEAAVHD MLAGNAFGDA 180
GHRIVIEEFL DGEEASFIVM VDGEHVLPMA TSQDHKRVGD KDTGPNTGGM GAYSPAPVVT 240
DDVHQRTMER IIWPTVKGMA AEGNTYTGFL YAGLMIDKQG NPKVIEFNCR FGDPETQPIM 300
LRMKSDLVEL CLAACESKLD EKTSEWDERA SLGVVMAAGG YPGDYRTGDV IHGLPLEEVA 360
GGKVFHAGTK LADDEQVVTN GGRVLCVTAL GHTVAEAQKR AYALMTDIHW DDCFCRKDIG 420
WRAIEREQN 429 
Gene Ontology
 GO:0005524; F:ATP binding; IDA:EcoliWiki.
 GO:0042623; F:ATPase activity, coupled; IDA:EcoliWiki.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0030145; F:manganese ion binding; IEA:HAMAP.
 GO:0004637; F:phosphoribosylamine-glycine ligase activity; IDA:EcoCyc.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro.
 GO:0006164; P:purine nucleotide biosynthetic process; NAS:EcoliWiki.
 GO:0006974; P:response to DNA damage stimulus; IEP:EcoliWiki. 
Interpro
 IPR011761; ATP-grasp.
 IPR013815; ATP_grasp_subdomain_1.
 IPR013816; ATP_grasp_subdomain_2.
 IPR016185; PreATP-grasp_dom.
 IPR020561; PRibGlycinamid_synth_ATP-grasp.
 IPR000115; PRibGlycinamide_synth.
 IPR020560; PRibGlycinamide_synth_C-dom.
 IPR020559; PRibGlycinamide_synth_CS.
 IPR020562; PRibGlycinamide_synth_N.
 IPR011054; Rudment_hybrid_motif. 
Pfam
 PF01071; GARS_A
 PF02843; GARS_C
 PF02844; GARS_N 
SMART
  
PROSITE
 PS50975; ATP_GRASP
 PS00184; GARS 
PRINTS