CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001470
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial import receptor subunit TOM70 
Protein Synonyms/Alias
 Mitochondrial precursor proteins import receptor; Translocase of outer membrane 70 kDa subunit 
Gene Name
 TOMM70A 
Gene Synonyms/Alias
 KIAA0719; TOM70 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
78RGDASGLKRNSERKTubiquitination[1]
120RAQAAKNKGNKYFKAubiquitination[1]
123AAKNKGNKYFKAGKYubiquitination[1]
148ISLCPTEKNVDLSTFubiquitination[1]
168AAFEQLQKWKEVAQDubiquitination[1, 2, 3, 4, 5, 6, 7]
170FEQLQKWKEVAQDCTubiquitination[1, 5]
178EVAQDCTKAVELNPKubiquitination[1]
185KAVELNPKYVKALFRacetylation[8, 9]
185KAVELNPKYVKALFRubiquitination[1]
230QSMLLADKVLKLLGKubiquitination[1]
233LLADKVLKLLGKEKAubiquitination[2, 6]
245EKAKEKYKNREPLMPubiquitination[4]
258MPSPQFIKSYFSSFTubiquitination[1]
275IISQPMLKGEKSDEDubiquitination[1, 2, 3, 6]
278QPMLKGEKSDEDKDKubiquitination[1]
293EGEALEVKENSGYLKubiquitination[3]
302NSGYLKAKQYMEEENubiquitination[1, 3]
326KEIDAEGKYMAEALLubiquitination[1, 5]
349IGNANAAKPDLDKVIubiquitination[1]
359LDKVISLKEANVKLRubiquitination[1, 3]
441SALAQAQKCFALYRQubiquitination[2, 6, 10]
463SQIQAAMKGFEEVIKubiquitination[1]
470KGFEEVIKKFPRCAEacetylation[8]
470KGFEEVIKKFPRCAEubiquitination[1]
524GLLQLQWKQDLDRGLubiquitination[1]
536RGLELISKAIEIDNKubiquitination[1, 3]
563VQRGNMEKAIDMFNKubiquitination[1]
570KAIDMFNKAINLAKSubiquitination[1]
604VAKKYGLKPPTL***ubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [9] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [10] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
 Receptor that accelerates the import of all mitochondrial precursor proteins (By similarity). 
Sequence Annotation
 REPEAT 114 147 TPR 1.
 REPEAT 153 186 TPR 2.
 REPEAT 294 327 TPR 3.
 REPEAT 329 362 TPR 4.
 REPEAT 367 400 TPR 5.
 REPEAT 401 434 TPR 6.
 REPEAT 440 475 TPR 7.
 REPEAT 476 509 TPR 8.
 REPEAT 511 544 TPR 9.
 REPEAT 545 578 TPR 10.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 85 85 Phosphothreonine (By similarity).
 MOD_RES 91 91 Phosphoserine.
 MOD_RES 185 185 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Membrane; Mitochondrion; Mitochondrion outer membrane; Phosphoprotein; Reference proteome; Repeat; TPR repeat; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 608 AA 
Protein Sequence
MAASKPVEAA VVAAAVPSSG SGVGGGGTAG PGTGGLPRWQ LALAVGAPLL LGAGAIYLWS 60
RQQRRREARG RGDASGLKRN SERKTPEGRA SPAPGSGHPE GPGAHLDMNS LDRAQAAKNK 120
GNKYFKAGKY EQAIQCYTEA ISLCPTEKNV DLSTFYQNRA AAFEQLQKWK EVAQDCTKAV 180
ELNPKYVKAL FRRAKAHEKL DNKKECLEDV TAVCILEGFQ NQQSMLLADK VLKLLGKEKA 240
KEKYKNREPL MPSPQFIKSY FSSFTDDIIS QPMLKGEKSD EDKDKEGEAL EVKENSGYLK 300
AKQYMEEENY DKIISECSKE IDAEGKYMAE ALLLRATFYL LIGNANAAKP DLDKVISLKE 360
ANVKLRANAL IKRGSMYMQQ QQPLLSTQDF NMAADIDPQN ADVYHHRGQL KILLDQVEEA 420
VADFDECIRL RPESALAQAQ KCFALYRQAY TGNNSSQIQA AMKGFEEVIK KFPRCAEGYA 480
LYAQALTDQQ QFGKADEMYD KCIDLEPDNA TTYVHKGLLQ LQWKQDLDRG LELISKAIEI 540
DNKCDFAYET MGTIEVQRGN MEKAIDMFNK AINLAKSEME MAHLYSLCDA AHAQTEVAKK 600
YGLKPPTL 608 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005742; C:mitochondrial outer membrane translocase complex; TAS:BHF-UCL.
 GO:0008320; F:protein transmembrane transporter activity; TAS:BHF-UCL.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0006626; P:protein targeting to mitochondrion; TAS:BHF-UCL. 
Interpro
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF00515; TPR_1
 PF13181; TPR_8 
SMART
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS