UniProt Accession

 PHOU_ECOLI; P0A9K7; P07656; Q2M842 

Genbank Protein ID

 X02723; K01992; L10328; U00096; AP009048; M16487 

Genbank Nucleotide ID

 CAA26510.1; AAA24382.1; AAA62075.1; AAC76747.1; BAE77564.1; AAA23507.1 

Protein Name

 Phosphate-specific transport system accessory protein PhoU 

Protein Synonyms/Alias

 Pst system accessory protein PhoU; Negative regulator of Pho regulon 

Gene Name

 phoU 

Gene Synonyms/Alias

 nmpA; b3724; JW3702 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
52	NQDSDLAKRVIEGDK	acetylation	[1]
59	KRVIEGDKNVNMMEV	acetylation	[1]
108	RIGDVADKICRTALE	acetylation	[1]
116	ICRTALEKFSQQHQP	acetylation	[1]
162	VRIYREDKKVDQEYE	acetylation	[1]
232	VGGDELDKLLAGKDS	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Part of the phosphate (Pho) regulon, which plays a key role in phosphate homeostasis. Encoded together with proteins of the phosphate-specific transport (Pst) system in the polycistronic pstSCAB-phoU operon. PhoU is essential for the repression of the Pho regulon at high phosphate conditions. In this role, it may bind, possibly as a chaperone, to PhoR, PhoB or a PhoR-PhoB complex to promote dephosphorylation of phospho-PhoB, or inhibit formation of the PhoR-PhoB transitory complex. Is also part of complex networks important for bacterial virulence, tolerance to antibiotics and stress response. 

Sequence Annotation

  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; Pharmaceutical; Phosphate transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 241 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0030643; P:cellular phosphate ion homeostasis; TAS:UniProtKB.
 GO:0071229; P:cellular response to acid; IMP:UniProtKB.
 GO:0071236; P:cellular response to antibiotic; IMP:UniProtKB.
 GO:0034605; P:cellular response to heat; IMP:UniProtKB.
 GO:0016036; P:cellular response to phosphate starvation; IEP:UniProtKB.
 GO:0010629; P:negative regulation of gene expression; IMP:UniProtKB.
 GO:0032413; P:negative regulation of ion transmembrane transporter activity; IMP:UniProtKB.
 GO:0045936; P:negative regulation of phosphate metabolic process; IMP:UniProtKB.
 GO:2000186; P:negative regulation of phosphate transmembrane transport; IMP:UniProtKB.
 GO:0009405; P:pathogenesis; IMP:UniProtKB.
 GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
 GO:0001558; P:regulation of cell growth; IMP:UniProtKB. 

Interpro

 IPR008170; PhoU.
 IPR026022; PhoU_dom. 

Pfam

 PF01895; PhoU 

SMART

  

PROSITE

  

PRINTS