CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023645
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 BTB/POZ domain-containing protein 3 
Protein Synonyms/Alias
  
Gene Name
 BTBD3 
Gene Synonyms/Alias
 KIAA0952 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
223LETSLSAKNACVLLSubiquitination[1]
309LALSIENKRKVLGKAubiquitination[1, 2, 3, 4]
315NKRKVLGKALYLIRIubiquitination[1, 3, 4, 5]
357LWYTAAKKPELQFVSubiquitination[4]
365PELQFVSKARKGLVPubiquitination[2, 4]
368QFVSKARKGLVPQRCubiquitination[4]
403SIQFAVDKRVFIAGFubiquitination[3]
Reference
 [1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
 DOMAIN 120 190 BTB.  
Keyword
 Alternative splicing; Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 522 AA 
Protein Sequence
MVDDKEKNMK CLTFFLMLPE TVKNRSKKSS KKANTSSSSS NSSKLPPVCY EIITLKTKKK 60
KMAADIFPRK KPANSSSTSV QQYHQQNLSN NNLIPAPNWQ GLYPTIRERN AMMFNNDLMA 120
DVHFVVGPPG GTQRLPGHKY VLAVGSSVFH AMFYGELAED KDEIRIPDVE PAAFLAMLKY 180
IYCDEIDLAA DTVLATLYAA KKYIVPHLAR ACVNFLETSL SAKNACVLLS QSCLFEEPDL 240
TQRCWEVIDA QAELALKSEG FCDIDFQTLE SILRRETLNA KEIVVFEAAL NWAEVECQRQ 300
DLALSIENKR KVLGKALYLI RIPTMALDDF ANGAAQSGVL TLNETNDIFL WYTAAKKPEL 360
QFVSKARKGL VPQRCHRFQS CAYRSNQWRY RGRCDSIQFA VDKRVFIAGF GLYGSSCGSA 420
EYSAKIELKR QGVVLGQNLS KYFSDGSSNT FPVWFEYPVQ IEPDTFYTAS VILDGNELSY 480
FGQEGMTEVQ CGKVTVQFQC SSDSTNGTGV QGGQIPELIF YA 522 
Gene Ontology
  
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR012983; PHR. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF08005; PHR 
SMART
 SM00875; BACK
 SM00225; BTB 
PROSITE
 PS50097; BTB 
PRINTS