CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005114
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Catechol O-methyltransferase 
Protein Synonyms/Alias
  
Gene Name
 Comt 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
171DLIPQLKKKYDVDTLacetylation[1]
172LIPQLKKKYDVDTLDacetylation[1]
252KVVDGLEKAIYQGPSacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. 
Sequence Annotation
 REGION 160 163 S-adenosyl-L-methionine binding.
 METAL 184 184 Magnesium.
 METAL 212 212 Magnesium.
 METAL 213 213 Magnesium.
 BINDING 85 85 S-adenosyl-L-methionine; via amide
 BINDING 115 115 S-adenosyl-L-methionine.
 BINDING 133 133 S-adenosyl-L-methionine.
 BINDING 184 184 S-adenosyl-L-methionine.
 BINDING 187 187 Substrate.
 BINDING 213 213 Substrate.
 BINDING 242 242 Substrate.  
Keyword
 3D-structure; Alternative initiation; Catecholamine metabolism; Cell membrane; Complete proteome; Cytoplasm; Magnesium; Membrane; Metal-binding; Methyltransferase; Neurotransmitter degradation; Reference proteome; S-adenosyl-L-methionine; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 264 AA 
Protein Sequence
MPLAAVSLGL LLLALLLLLR HLGWGLVTIF WFEYVLQPVH NLIMGDTKEQ RILRYVQQNA 60
KPGDPQSVLE AIDTYCTQKE WAMNVGDAKG QIMDAVIREY SPSLVLELGA YCGYSAVRMA 120
RLLQPGARLL TMEMNPDYAA ITQQMLNFAG LQDKVTILNG ASQDLIPQLK KKYDVDTLDM 180
VFLDHWKDRY LPDTLLLEKC GLLRKGTVLL ADNVIVPGTP DFLAYVRGSS SFECTHYSSY 240
LEYMKVVDGL EKAIYQGPSS PDKS 264 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0016020; C:membrane; IDA:RGD.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0016206; F:catechol O-methyltransferase activity; IDA:RGD.
 GO:0000287; F:magnesium ion binding; TAS:RGD.
 GO:0042420; P:dopamine catabolic process; IEA:Compara.
 GO:0042417; P:dopamine metabolic process; TAS:RGD.
 GO:0008210; P:estrogen metabolic process; IMP:RGD.
 GO:0007565; P:female pregnancy; IMP:RGD.
 GO:0007612; P:learning; IMP:RGD.
 GO:0048609; P:multicellular organismal reproductive process; IMP:RGD.
 GO:0045963; P:negative regulation of dopamine metabolic process; IMP:RGD.
 GO:0048662; P:negative regulation of smooth muscle cell proliferation; IMP:RGD.
 GO:0042135; P:neurotransmitter catabolic process; IEA:UniProtKB-KW.
 GO:0050668; P:positive regulation of homocysteine metabolic process; IMP:RGD.
 GO:0042493; P:response to drug; IEP:RGD.
 GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
 GO:0014070; P:response to organic cyclic compound; IEP:RGD.
 GO:0048265; P:response to pain; IMP:RGD.
 GO:0046498; P:S-adenosylhomocysteine metabolic process; TAS:RGD.
 GO:0046500; P:S-adenosylmethionine metabolic process; TAS:RGD. 
Interpro
 IPR025782; Catechol_O-MeTrfase.
 IPR017128; Catechol_O-MeTrfase_euk.
 IPR002935; O-MeTrfase_3. 
Pfam
 PF01596; Methyltransf_3 
SMART
  
PROSITE
  
PRINTS