CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006489
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cystathionine beta-synthase 
Protein Synonyms/Alias
 Beta-thionase; Serine sulfhydrase 
Gene Name
 CBS 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
82KILPDILKKIGDTPMubiquitination[1]
102IGKKFGLKCELLAKCubiquitination[1]
119FNAGGSVKDRISLRMubiquitination[1]
137AERDGTLKPGDTIIEubiquitination[1]
177PEKMSSEKVDVLRALubiquitination[1]
211VGVAWRLKNEIPNSHubiquitination[1]
322LDRTVVDKWFKSNDEubiquitination[1]
325TVVDKWFKSNDEEAFubiquitination[1]
384SVRNYMTKFLSDRWMubiquitination[1]
394SDRWMLQKGFLKEEDubiquitination[2]
481QPSDQVGKVIYKQFKubiquitination[1]
485QVGKVIYKQFKQIRLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury (By similarity). 
Sequence Annotation
 DOMAIN 418 476 CBS.
 REGION 256 260 Pyridoxal phosphate binding.
 METAL 52 52 Iron (heme axial ligand).
 METAL 65 65 Iron (heme axial ligand).
 BINDING 149 149 Pyridoxal phosphate.
 BINDING 349 349 Pyridoxal phosphate.
 MOD_RES 27 27 Phosphoserine.
 MOD_RES 119 119 N6-(pyridoxal phosphate)lysine.
 CROSSLNK 211 211 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Allosteric enzyme; Alternative splicing; Amino-acid biosynthesis; CBS domain; Complete proteome; Cysteine biosynthesis; Cytoplasm; Disease mutation; Heme; Iron; Isopeptide bond; Lyase; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Pyridoxal phosphate; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 551 AA 
Protein Sequence
MPSETPQAEV GPTGCPHRSG PHSAKGSLEK GSPEDKEAKE PLWIRPDAPS RCTWQLGRPA 60
SESPHHHTAP AKSPKILPDI LKKIGDTPMV RINKIGKKFG LKCELLAKCE FFNAGGSVKD 120
RISLRMIEDA ERDGTLKPGD TIIEPTSGNT GIGLALAAAV RGYRCIIVMP EKMSSEKVDV 180
LRALGAEIVR TPTNARFDSP ESHVGVAWRL KNEIPNSHIL DQYRNASNPL AHYDTTADEI 240
LQQCDGKLDM LVASVGTGGT ITGIARKLKE KCPGCRIIGV DPEGSILAEP EELNQTEQTT 300
YEVEGIGYDF IPTVLDRTVV DKWFKSNDEE AFTFARMLIA QEGLLCGGSA GSTVAVAVKA 360
AQELQEGQRC VVILPDSVRN YMTKFLSDRW MLQKGFLKEE DLTEKKPWWW HLRVQELGLS 420
APLTVLPTIT CGHTIEILRE KGFDQAPVVD EAGVILGMVT LGNMLSSLLA GKVQPSDQVG 480
KVIYKQFKQI RLTDTLGRLS HILEMDHFAL VVHEQIQSQD QAWAGVVGGP ADHSTGKSSQ 540
RQMVFGVVTA IDLLNFVAAQ ERDQK 565 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0004122; F:cystathionine beta-synthase activity; IDA:UniProtKB.
 GO:0020037; F:heme binding; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0072341; F:modified amino acid binding; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0030170; F:pyridoxal phosphate binding; IDA:UniProtKB.
 GO:0001974; P:blood vessel remodeling; IEA:Compara.
 GO:0060351; P:cartilage development involved in endochondral bone morphogenesis; IEA:Compara.
 GO:0034641; P:cellular nitrogen compound metabolic process; TAS:Reactome.
 GO:0071456; P:cellular response to hypoxia; IEA:Compara.
 GO:0021587; P:cerebellum morphogenesis; IEA:Compara.
 GO:0006535; P:cysteine biosynthetic process from serine; IEA:InterPro.
 GO:0019343; P:cysteine biosynthetic process via cystathionine; IEA:InterPro.
 GO:0001958; P:endochondral ossification; IEA:Compara.
 GO:0043418; P:homocysteine catabolic process; IDA:UniProtKB.
 GO:0070814; P:hydrogen sulfide biosynthetic process; IDA:UniProtKB.
 GO:0019448; P:L-cysteine catabolic process; IDA:UniProtKB.
 GO:0006565; P:L-serine catabolic process; IDA:UniProtKB.
 GO:0060135; P:maternal process involved in female pregnancy; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0050880; P:regulation of blood vessel size; IEA:Compara.
 GO:0030823; P:regulation of cGMP metabolic process; IEA:Compara.
 GO:0043506; P:regulation of JUN kinase activity; IEA:Compara.
 GO:0051593; P:response to folic acid; IEA:Compara.
 GO:0006801; P:superoxide metabolic process; IEA:Compara.
 GO:0019346; P:transsulfuration; TAS:Reactome. 
Interpro
 IPR001216; Cys_synth_BS.
 IPR005857; Cysta_beta_synth.
 IPR000644; Cysta_beta_synth_core.
 IPR001926; Trp_syn_b_sub_like_PLP_eny_SF. 
Pfam
 PF00571; CBS
 PF00291; PALP 
SMART
 SM00116; CBS 
PROSITE
 PS51371; CBS
 PS00901; CYS_SYNTHASE 
PRINTS