CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044775
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 cAMP-dependent protein kinase type I-alpha regulatory subunit 
Protein Synonyms/Alias
  
Gene Name
 PRKAR1A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
24ECELYVQKHNIQALLubiquitination[1, 2, 3]
59EYFERLEKEEAKQIQubiquitination[2, 3]
63RLEKEEAKQIQNLQKubiquitination[2, 3, 4, 5]
70KQIQNLQKAGTRTDSubiquitination[1, 2, 3, 4, 5]
92PPPNPVVKGRRRRGAubiquitination[1, 2, 3, 4, 5, 6]
123KVIPKDYKTMAALAKubiquitination[2, 4]
130KTMAALAKAIEKNVLubiquitination[2]
134ALAKAIEKNVLFSHLubiquitination[1, 7]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 168 AA 
Protein Sequence
MESGSTAASE EARSLRECEL YVQKHNIQAL LKDSIVQLCT ARPERPMAFL REYFERLEKE 60
EAKQIQNLQK AGTRTDSRED EISPPPPNPV VKGRRRRGAI SAEVYTEEDA ASYVRKVIPK 120
DYKTMAALAK AIEKNVLFSH LDDNERSDIF DAMFSVSFIA GETVIQQG 168 
Gene Ontology
 GO:0005952; C:cAMP-dependent protein kinase complex; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0031594; C:neuromuscular junction; IEA:Compara.
 GO:0030552; F:cAMP binding; IEA:Compara.
 GO:0008603; F:cAMP-dependent protein kinase regulator activity; IEA:Compara.
 GO:0060038; P:cardiac muscle cell proliferation; IEA:Compara.
 GO:0007143; P:female meiosis; IEA:Compara.
 GO:0001707; P:mesoderm formation; IEA:Compara.
 GO:0045835; P:negative regulation of meiosis; IEA:Compara.
 GO:0006469; P:negative regulation of protein kinase activity; IEA:Compara.
 GO:0045214; P:sarcomere organization; IEA:Compara.
 GO:0007165; P:signal transduction; IEA:InterPro. 
Interpro
 IPR003117; cAMP_dep_PK_reg_su_I/II_a/b.
 IPR018490; cNMP-bd-like.
 IPR000595; cNMP-bd_dom.
 IPR014710; RmlC-like_jellyroll. 
Pfam
 PF02197; RIIa 
SMART
 SM00394; RIIa 
PROSITE
 PS50042; CNMP_BINDING_3 
PRINTS