CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024238
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 S-adenosylmethionine synthase 
Protein Synonyms/Alias
 AdoMet synthase; MAT; Methionine adenosyltransferase 
Gene Name
 metK 
Gene Synonyms/Alias
 MSMEG_3055; MSMEI_2979 
Created Date
 July 27, 2013 
Organism
 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
NCBI Taxa ID
 246196 
Lysine Modification
Position
Peptide
Type
References
341SETVDPAKIEKAIGEpupylation[1]
396TNKVDDLKSAI****pupylation[2]
Reference
 [1] Expansion of the mycobacterial "PUPylome".
 Watrous J, Burns K, Liu WT, Patel A, Hook V, Bafna V, Barry CE 3rd, Bark S, Dorrestein PC.
 Mol Biosyst. 2010 Feb;6(2):376-85. [PMID: 20094657]
 [2] Proteome-wide identification of mycobacterial pupylation targets.
 Poulsen C, Akhter Y, Jeon AH, Schmitt-Ulms G, Meyer HE, Stefanski A, Stühler K, Wilmanns M, Song YH.
 Mol Syst Biol. 2010 Jul 13;6:386. [PMID: 20631680] 
Functional Description
 Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme (By similarity). 
Sequence Annotation
 NP_BIND 276 283 ATP (Potential).
 METAL 18 18 Magnesium (By similarity).
 METAL 44 44 Potassium (By similarity).
 METAL 280 280 Potassium (By similarity).
 METAL 288 288 Magnesium (By similarity).
 CROSSLNK 341 341 Isoglutamyl lysine isopeptide (Lys-Gln)  
Keyword
 ATP-binding; Cobalt; Complete proteome; Cytoplasm; Isopeptide bond; Magnesium; Metal-binding; Nucleotide-binding; One-carbon metabolism; Potassium; Reference proteome; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 399 AA 
Protein Sequence
MSKGRLFTSE SVTEGHPDKI CDAISDSVLD ALLEQDPKSR VAVETLVTTG QVHVAGEVTT 60
TAYADIPKIV RDRILDIGYD SSTKGFDGAS CGVNVAIGAQ SPDIAQGVDT AHETRVEGKA 120
DPLDLQGAGD QGLMFGYAIG DTPELMPLPI ALAHRLARRL TEVRKNGVLD YLRPDGKTQV 180
TIQYDGTTPV RLDTVVLSTQ HADGIDLEGT LTPDIREKVV NTVLADLGHE TLDTSDYRLL 240
VNPTGKFVLG GPMGDAGLTG RKIIVDTYGG WARHGGGAFS GKDPSKVDRS AAYAMRWVAK 300
NVVAAGLAER VEVQVAYAIG KAAPVGLFVE TFGSETVDPA KIEKAIGEVF DLRPAAIVRD 360
LDLLRPIYAP TAAYGHFGRT DIELPWEQTN KVDDLKSAI 399 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0004478; F:methionine adenosyltransferase activity; IEA:HAMAP.
 GO:0006730; P:one-carbon metabolic process; IEA:HAMAP.
 GO:0006556; P:S-adenosylmethionine biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR022631; ADOMET_SYNTHASE_CS.
 IPR022630; S-AdoMet_synt_C.
 IPR022629; S-AdoMet_synt_central.
 IPR022628; S-AdoMet_synt_N.
 IPR002133; S-AdoMet_synthetase.
 IPR022636; S-AdoMet_synthetase_sfam. 
Pfam
 PF02773; S-AdoMet_synt_C
 PF02772; S-AdoMet_synt_M
 PF00438; S-AdoMet_synt_N 
SMART
  
PROSITE
 PS00376; ADOMET_SYNTHASE_1
 PS00377; ADOMET_SYNTHASE_2 
PRINTS