CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020888
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin-like modifier-activating enzyme ATG7 
Protein Synonyms/Alias
 ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; mAGP7; Ubiquitin-activating enzyme E1-like protein 
Gene Name
 Atg7 
Gene Synonyms/Alias
 Apg7l 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
419AAAERLQKIFPGVNAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. 
Sequence Annotation
 ACT_SITE 567 567 Glycyl thioester intermediate (Probable).  
Keyword
 Autophagy; Complete proteome; Cytoplasm; Protein transport; Reference proteome; Transport; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 698 AA 
Protein Sequence
MGDPGLAKLQ FAPFNSALDV GFWHELTQKK LNEYRLDEAP KDIKGYYYNG DSAGLPTRLT 60
LEFSAFDMSA STPAHCCPAM GTLHNTNTLE AFKTADKKLL LEQSANEIWE AIKSGAALEN 120
PMLLNKFLLL TFADLKKYHF YYWFCCPALC LPESIPLIRG PVSLDQRLSP KQIQALEHAY 180
DDLCRAEGVT ALPYFLFKYD DDTVLVSLLK HYSDFFQGQR TKITVGVYDP CNLAQYPGWP 240
LRNFLVLAAH RWSGSFQSVE VLCFRDRTMQ GARDVTHSII FEVKLPEMAF SPDCPKAVGW 300
EKNQKGGMGP RMVNLSGCMD PKRLAESSVD LNLKLMCWRL VPTLDLDKVV SVKCLLLGAG 360
TLGCNVARTL MGWGVRHVTF VDNAKISYSN PVRQPLYEFE DCLGGGKPKA LAAAERLQKI 420
FPGVNARGFN MSIPMPGHPV NFSDVTMEQA RRDVEQLEQL IDNHDVIFLL MDTRESRWLP 480
TVIAASKRKL VINAALGFDT FVVMRHGLKK PKQQGAGDLC PSHLVAPADL GSSLFANIPG 540
YKLGCYFCND VVAPGDSTRD RTLDQQCTVS RPGLAVIAGA LAVELMVSVL QHPEGGYAIA 600
SSSDDRMNEP PTSLGLVPHQ IRGFLSRFDN VLPVSLAFDK CTACSPKVLD QYEREGFTFL 660
AKVFNSSHSF LEDLTGLTLL HQETQAAEIW DMSDEETV 698 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0019778; F:APG12 activating enzyme activity; IMP:UniProtKB.
 GO:0004839; F:ubiquitin activating enzyme activity; IDA:MGI.
 GO:0007628; P:adult walking behavior; IMP:MGI.
 GO:0006914; P:autophagy; IMP:MGI.
 GO:0055013; P:cardiac muscle cell development; IMP:MGI.
 GO:0006520; P:cellular amino acid metabolic process; IMP:MGI.
 GO:0016044; P:cellular membrane organization; IMP:MGI.
 GO:0021955; P:central nervous system neuron axonogenesis; IMP:MGI.
 GO:0021680; P:cerebellar Purkinje cell layer development; IMP:MGI.
 GO:0021987; P:cerebral cortex development; IMP:MGI.
 GO:0001889; P:liver development; IMP:MGI.
 GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
 GO:0050877; P:neurological system process; IMP:MGI.
 GO:0006996; P:organelle organization; IMP:MGI.
 GO:0016239; P:positive regulation of macroautophagy; IEA:Compara.
 GO:0031401; P:positive regulation of protein modification process; IDA:MGI.
 GO:0009791; P:post-embryonic development; IMP:MGI.
 GO:0030163; P:protein catabolic process; IMP:MGI.
 GO:0006497; P:protein lipidation; ISO:MGI.
 GO:0032446; P:protein modification by small protein conjugation; IMP:MGI.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0021860; P:pyramidal neuron development; IMP:MGI.
 GO:0031396; P:regulation of protein ubiquitination; IMP:MGI.
 GO:0042594; P:response to starvation; IMP:MGI. 
Interpro
 IPR006285; Atg7.
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd. 
Pfam
 PF00899; ThiF 
SMART
  
PROSITE
  
PRINTS