| Tag | Content |
|---|
| CPLM ID | CPLM-002838 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Homothallic switching endonuclease |
Protein Synonyms/Alias | Ho endonuclease |
Gene Name | HO |
Gene Synonyms/Alias | YDL227C |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 46 | NVTQGYQKIYNIQQK | ubiquitination | [1] | | 53 | KIYNIQQKTKHRAFE | ubiquitination | [1] | | 516 | ARCYGRYKFSGHHCI | ubiquitination | [1] | | 554 | TPEGLPVKGPECIKC | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Initiation of mating type interconversion. This protein is a site-specific endonuclease that cleaves a site in the mat locus on chromosome III. The double-strand break is followed by a unidirectional gene conversion event that replaces the information at the mat locus by information copied from either of the two homologous loci (HMR and HML) that reside at the extremity of the chromosome III. Endonuclease expression takes place in late G1 just before cells enter S phase. |
Sequence Annotation | DOMAIN 215 370 DOD-type homing endonuclease. |
Keyword | Complete proteome; DNA-binding; Endonuclease; Hydrolase; Metal-binding; Nuclease; Nucleus; Reference proteome; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 586 AA |
Protein Sequence | MLSENTTILM ANGEIKDIAN VTANSYVMCA DGSAARVINV TQGYQKIYNI QQKTKHRAFE 60
GEPGRLDPRR RTVYQRLALQ CTAGHKLSVR VPTKPLLEKS GRNATKYKVR WRNLQQCQTL 120
DGRIIIIPKN HHKTFPMTVE GEFAAKRFIE EMERSKGEYF NFDIEVRDLD YLDAQLRISS 180
CIRFGPVLAG NGVLSKFLTG RSDLVTPAVK SMAWMLGLWL GDSTTKEPEI SVDSLDPKLM 240
ESLRENAKIW GLYLTVCDDH VPLRAKHVRL HYGDGPDENR KTRNLRKNNP FWKAVTILKF 300
KRDLDGEKQI PEFMYGEHIE VREAFLAGLI DSDGYVVKKG EGPESYKIAI QTVYSSIMDG 360
IVHISRSLGM SATVTTRSAR EEIIEGRKVQ CQFTYDCNVA GGTTSQNVLS YCRSGHKTRE 420
VPPIIKREPV YFSFTDDFQG ESTVYGLTIE GHKNFLLGNK IEVKSCRGCC VGEQLKISQK 480
KNLKHCVACP RKGIKYFYKD WSGKNRVCAR CYGRYKFSGH HCINCKYVPE AREVKKAKDK 540
GEKLGITPEG LPVKGPECIK CGGILQFDAV RGPHKSCGNN AGARIC 586 |
Gene Ontology | GO:0005634; C:nucleus; IDA:SGD. GO:0003677; F:DNA binding; IEA:UniProtKB-KW. GO:0004519; F:endonuclease activity; IDA:SGD. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0000728; P:gene conversion at mating-type locus, DNA double-strand break formation; IDA:SGD. GO:0016539; P:intein-mediated protein splicing; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |