CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037298
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Mitotic checkpoint serine/threonine-protein kinase BUB1 
Protein Synonyms/Alias
  
Gene Name
 BUB1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
175LNQMITSKSNPGNNMubiquitination[1, 2, 3, 4]
187NNMACISKNQGSELSubiquitination[1, 3]
203VISSACDKESNMERRubiquitination[3, 4]
216RRVITISKSEYSVHSubiquitination[3]
241VVMYCKEKLIRGESEubiquitination[3]
264QKYNQRRKHEQWVNEubiquitination[3, 4]
279DRHYMKRKEANAFEEubiquitination[3, 4]
290AFEEQLLKQKMDELHubiquitination[3]
299KMDELHKKLHQVVETubiquitination[2, 3, 4]
353QTPVNMEKNPREAPPubiquitination[3, 4]
403SMFAVASKDAGCVNKubiquitination[3, 4]
410KDAGCVNKSTHEFKPubiquitination[4]
455MVQATPSKVQPSPTVubiquitination[3, 4]
535HVFEDGNKENYGLPQubiquitination[3, 4]
564SVSRLPSKPKEEVPHubiquitination[3]
566SRLPSKPKEEVPHAEubiquitination[3]
588VWGIRCNKTLAPSPKubiquitination[3, 4]
653VVPSRDGKFSPIQEKubiquitination[3, 4]
660KFSPIQEKSPKQALSubiquitination[3, 4]
663PIQEKSPKQALSSHMubiquitination[3]
756KLLSGLSKPVSSYPNubiquitination[1, 3, 4]
777KLPAIKPKTEFQLGSubiquitination[3]
815DLNDAKNKQKFVLKVubiquitination[3]
901IDMKLFPKGTIFTAKubiquitination[4]
950FGTYMKVKNEGGECKubiquitination[4]
957KNEGGECKPEGLFRRubiquitination[4]
999LLRQKLKKVFQQHYTubiquitination[3]
1008FQQHYTNKIRALRNRubiquitination[4]
1024IVLLLECKRSRK***ubiquitination[3]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1028 AA 
Protein Sequence
MDTPENVLQM LEAHMQSYKG NDPLGEWERY IQWVEENFPE NKEYLITLLE HLMKEFLDKK 60
KYHNDPRFIS YCLKFAEYNS DLHQFFEFLY NHGIGTLSSP LYIAWAGHLE AQGELQHASA 120
VLQRGIQNQA EPREFLQQQY RLFQTRLTET HLPAQARTSE PLHNVQVLNQ MITSKSNPGN 180
NMACISKNQG SELSGVISSA CDKESNMERR VITISKSEYS VHSSLASKVD VEQVVMYCKE 240
KLIRGESEFS FEELRAQKYN QRRKHEQWVN EDRHYMKRKE ANAFEEQLLK QKMDELHKKL 300
HQVVETSHED LPASQERSEV NPARMGPSVG SQQELRAPCL PVTYQQTPVN MEKNPREAPP 360
VVPPLANAIS AALVSPATSQ SIAPPVPLKA QTVTDSMFAV ASKDAGCVNK STHEFKPQSG 420
AEIKEGCETH KVANTSSFHT TPNTSLGMVQ ATPSKVQPSP TVHTKEALGF IMNMFQAPTL 480
PDISDDKDEW QSLDQNEDAF EAQFQKNVRS SGAWGVNKII SSLSSAFHVF EDGNKENYGL 540
PQPKNKPTGA RTFGERSVSR LPSKPKEEVP HAEEFLDDST VWGIRCNKTL APSPKSPGDF 600
TSAAQLASTP FHKLPVESVH ILEDKENVVA KQCTQATLDS CEENMVVPSR DGKFSPIQEK 660
SPKQALSSHM YSASLLRLSQ PAAGGVLTCE AELGVEACRL TDTDAAIAED PPDAIAGLQA 720
EWMQMSSLGT VDAPNFIVGN PWDDKLIFKL LSGLSKPVSS YPNTFEWQCK LPAIKPKTEF 780
QLGSKLVYVH HLLGEGAFAQ VYEATQGDLN DAKNKQKFVL KVQKPANPWE FYIGTQLMER 840
LKPSMQHMFM KFYSAHLFQN GSVLVGELYS YGTLLDDEDD LSAGLALIDL GQSIDMKLFP 900
KGTIFTAKCE TSGFQCVEML SNKPWNYQID YFGVAATVYC MLFGTYMKVK NEGGECKPEG 960
LFRRLPHLDM WNEFFHVMLN IPDCHHLPSL DLLRQKLKKV FQQHYTNKIR ALRNRLIVLL 1020
LECKRSRK 1028 
Gene Ontology
 GO:0016772; F:transferase activity, transferring phosphorus-containing groups; IEA:InterPro. 
Interpro
 IPR015661; Bub1/Mad3.
 IPR011009; Kinase-like_dom.
 IPR013212; Mad3_BUB1_I. 
Pfam
 PF08311; Mad3_BUB1_I 
SMART
 SM00777; Mad3_BUB1_I 
PROSITE
 PS51489; BUB1_N 
PRINTS