UniProt Accession

 GBLP_YEAST; P38011; D6VZT9; Q6LAA5 

Genbank Protein ID

 Z49702; Z49702; BK006946 

Genbank Nucleotide ID

 CAA89753.1; CAA89754.1; DAA10013.1 

Protein Name

 Guanine nucleotide-binding protein subunit beta-like protein 

Protein Synonyms/Alias

 Receptor for activated C kinase; Receptor of activated protein kinase C 1; RACK1 

Gene Name

 ASC1 

Gene Synonyms/Alias

 CPC2; YMR116C; YM9718.15C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
40	LLSASRDKTLISWKL	acetylation	[1]
46	DKTLISWKLTGDDQK	acetylation	[1]
46	DKTLISWKLTGDDQK	ubiquitination	[2, 3]
53	KLTGDDQKFGVPVRS	acetylation	[1]
53	KLTGDDQKFGVPVRS	ubiquitination	[2, 3]
87	ALSASWDKTLRLWDV	ubiquitination	[3]
107	YQRFVGHKSDVMSVD	acetylation	[1]
107	YQRFVGHKSDVMSVD	ubiquitination	[2]
117	VMSVDIDKKASMIIS	acetylation	[1]
117	VMSVDIDKKASMIIS	ubiquitination	[2, 3]
118	MSVDIDKKASMIISG	ubiquitination	[3]
132	GSRDKTIKVWTIKGQ	acetylation	[1]
132	GSRDKTIKVWTIKGQ	ubiquitination	[3]
137	TIKVWTIKGQCLATL	ubiquitination	[2, 3]
161	VRVVPNEKADDDSVT	acetylation	[1]
161	VRVVPNEKADDDSVT	ubiquitination	[2, 3]
176	IISAGNDKMVKAWNL	acetylation	[1]
176	IISAGNDKMVKAWNL	ubiquitination	[2, 3]
229	LWNLAAKKAMYTLSA	ubiquitination	[3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Located at the head of the 40S ribosomal subunit in the vicinity of the mRNA exit channel, it serves as a scaffold protein that can recruit other proteins to the ribosome. Involved in the negative regulation of translation of a specific subset of proteins. 

Sequence Annotation

 REPEAT 15 55 WD 1.
 REPEAT 63 102 WD 2.
 REPEAT 105 145 WD 3.
 REPEAT 147 191 WD 4.
 REPEAT 194 233 WD 5.
 REPEAT 235 275 WD 6.
 REPEAT 284 319 WD 7.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 96 96 Phosphothreonine.
 MOD_RES 168 168 Phosphothreonine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Reference proteome; Repeat; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 319 AA 

Protein Sequence

Gene Ontology

 GO:0022627; C:cytosolic small ribosomal subunit; IDA:SGD.
 GO:0005092; F:GDP-dissociation inhibitor activity; IDA:SGD.
 GO:0004871; F:signal transducer activity; IMP:SGD.
 GO:0007186; P:G-protein coupled receptor signaling pathway; IMP:SGD.
 GO:0010255; P:glucose mediated signaling pathway; IMP:SGD.
 GO:0001403; P:invasive growth in response to glucose limitation; IMP:SGD.
 GO:0017148; P:negative regulation of translation; IMP:SGD. 

Interpro

 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS

 PR00320; GPROTEINBRPT.