CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014731
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 HAUS augmin-like complex subunit 3 
Protein Synonyms/Alias
  
Gene Name
 HAUS3 
Gene Synonyms/Alias
 C4orf15 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
12NEFVETLKKIGYPKAubiquitination[1]
66EAFSILQKSGKPILEubiquitination[1]
69SILQKSGKPILEGAAubiquitination[1]
111DEVQTLLKLKNLKIQubiquitination[2]
219AALTLYTKKQFFQGIubiquitination[2, 3]
291SSMKSSIKWAEESLHubiquitination[1]
303SLHSLTSKAVDKENLubiquitination[2]
313DKENLDAKISSLTSEubiquitination[2]
323SLTSEIMKLEKEVTQubiquitination[1]
326SEIMKLEKEVTQIKDubiquitination[1]
332EKEVTQIKDRSLPAVubiquitination[1]
362DFDLQIAKQDYYTARubiquitination[2, 3]
379LVLNQLIKQKASFELubiquitination[1, 2, 4, 5]
444PRNTIDTKDYSTHRLubiquitination[1]
462LEGENKKKELFLTHGubiquitination[1]
479EEVAEKLKQNISLVQubiquitination[1]
502EHSFFLSKRNKDVDMubiquitination[1]
554TDILADVKTKRKTLAubiquitination[1, 2]
564RKTLANNKLHQMEREubiquitination[1, 2, 4, 5, 6]
584LKDEDYLKDIVENLEubiquitination[2]
597LETQSKIKAVSLED*ubiquitination[1, 2, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. 
Sequence Annotation
 MOD_RES 2 2 N-acetylserine.  
Keyword
 Acetylation; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Mitosis; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 603 AA 
Protein Sequence
MSCGNEFVET LKKIGYPKAD NLNGEDFDWL FEGVEDESFL KWFCGNVNEQ NVLSERELEA 60
FSILQKSGKP ILEGAALDEA LKTCKTSDLK TPRLDDKELE KLEDEVQTLL KLKNLKIQRR 120
NKCQLMASVT SHKSLRLNAK EEEATKKLKQ SQGILNAMIT KISNELQALT DEVTQLMMFF 180
RHSNLGQGTN PLVFLSQFSL EKYLSQEEQS TAALTLYTKK QFFQGIHEVV ESSNEDNFQL 240
LDIQTPSICD NQEILEERRL EMARLQLAYI CAQHQLIHLK ASNSSMKSSI KWAEESLHSL 300
TSKAVDKENL DAKISSLTSE IMKLEKEVTQ IKDRSLPAVV RENAQLLNMP VVKGDFDLQI 360
AKQDYYTARQ ELVLNQLIKQ KASFELLQLS YEIELRKHRD IYRQLENLVQ ELSQSNMMLY 420
KQLEMLTDPS VSQQINPRNT IDTKDYSTHR LYQVLEGENK KKELFLTHGN LEEVAEKLKQ 480
NISLVQDQLA VSAQEHSFFL SKRNKDVDML CDTLYQGGNQ LLLSDQELTE QFHKVESQLN 540
KLNHLLTDIL ADVKTKRKTL ANNKLHQMER EFYVYFLKDE DYLKDIVENL ETQSKIKAVS 600
LED 603 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0070652; C:HAUS complex; IDA:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0051297; P:centrosome organization; IMP:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0051225; P:spindle assembly; IMP:UniProtKB. 
Interpro
 IPR026206; HAUS3. 
Pfam
  
SMART
  
PROSITE
  
PRINTS
 PR02089; HAUSAUGMINL3.