CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-027591
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lysine--tRNA ligase 
Protein Synonyms/Alias
 Lysyl-tRNA synthetase 
Gene Name
 Kars 
Gene Synonyms/Alias
 mCG_141545 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
64AEKKLAEKEAKQKELacetylation[1]
105LDPNQYYKIRSQAVQacetylation[2]
105LDPNQYYKIRSQAVQubiquitination[3]
168AKRASGGKLIFYDLRacetylation[4, 5]
276QKFIVRSKIITYIRSacetylation[5]
276QKFIVRSKIITYIRSsuccinylation[5]
276QKFIVRSKIITYIRSubiquitination[3]
332IAPELYHKMLVVGGIacetylation[4]
332IAPELYHKMLVVGGIubiquitination[3]
390KMLSGMVKSITGSYKacetylation[4]
390KMLSGMVKSITGSYKubiquitination[3]
434LEKALGVKLPETSLFubiquitination[3]
448FETEETRKILDDICVubiquitination[3]
519RFELFVMKKEICNAYacetylation[4]
519RFELFVMKKEICNAYubiquitination[3]
520FELFVMKKEICNAYTacetylation[4]
520FELFVMKKEICNAYTubiquitination[3]
592LTDSNNIKEVLLFPAubiquitination[3]
605PAMKPEDKKETAATTubiquitination[3]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 624 AA 
Protein Sequence
MLMQAAVRLV RGALRQTSWA EWGQRELRLG HLAPFTTLHK DQPLSDRRSE LKRRLKAEKK 60
LAEKEAKQKE LSEKQLNQTA SAPNHTADNG VGAEEETLDP NQYYKIRSQA VQQLKVTGED 120
PYPHKFHVDI SLTQFIQEYS HLQPGDHLTD VTLKVAGRIH AKRASGGKLI FYDLRGEGVK 180
LQVMANSRNY KSEEEFVHIN NKLRRGDIIG VEGNPGKTKK GELSIIPQEI TLLSPCLHML 240
PHLHFGLKDK ETRYRQRYLD LILNDFVRQK FIVRSKIITY IRSFLDELGF LEIETPMMNI 300
IPGGAVAKPF ITYHNELDMN LYMRIAPELY HKMLVVGGID RVYEIGRQFR NEGIDLTHNP 360
EFTTCEFYMA YADYHDLMEI TEKMLSGMVK SITGSYKITY HPDGPEGQAY EVDFTPPFRR 420
ISMVEELEKA LGVKLPETSL FETEETRKIL DDICVAKAVE CPPPRTTARL LDKLVGEFLE 480
VTCISPTFIC DHPQIMSPLA KWHRSKEGLT ERFELFVMKK EICNAYTELN DPVRQRQLFE 540
EQAKAKAAGD DEAMFIDENF CTALEYGLPP TAGWGMGIDR LTMFLTDSNN IKEVLLFPAM 600
KPEDKKETAA TTETPESTEA SPSV 624 
Gene Ontology
 GO:0017101; C:aminoacyl-tRNA synthetase multienzyme complex; IEA:Compara.
 GO:0015630; C:microtubule cytoskeleton; IEA:Compara.
 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0016597; F:amino acid binding; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:Compara.
 GO:0004824; F:lysine-tRNA ligase activity; ISS:UniProtKB.
 GO:0000049; F:tRNA binding; IEA:Compara.
 GO:0015966; P:diadenosine tetraphosphate biosynthetic process; IEA:Compara.
 GO:0006430; P:lysyl-tRNA aminoacylation; ISS:UniProtKB. 
Interpro
 IPR004364; aa-tRNA-synt_II.
 IPR018150; aa-tRNA-synt_II-like.
 IPR006195; aa-tRNA-synth_II.
 IPR002313; Lys-tRNA-ligase_II.
 IPR018149; Lys-tRNA-synth_II_C.
 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase. 
Pfam
 PF00152; tRNA-synt_2
 PF01336; tRNA_anti 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS
 PR00982; TRNASYNTHLYS.