CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-028342
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha actinin 1a 
Protein Synonyms/Alias
 Alpha-actinin-1 
Gene Name
 Actn1 
Gene Synonyms/Alias
 Actn1a 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
31LLDPAWEKQQRKTFTubiquitination[1]
35AWEKQQRKTFTAWCNacetylation[2]
35AWEKQQRKTFTAWCNubiquitination[1]
95HKISNVNKALDFIASacetylation[2]
103ALDFIASKGVKLVSIacetylation[2, 3]
103ALDFIASKGVKLVSIsuccinylation[3]
106FIASKGVKLVSIGAEubiquitination[1]
162QRKTAPYKNVNIQNFacetylation[2]
195PELIDYGKLRKDDPLacetylation[2, 4]
195PELIDYGKLRKDDPLubiquitination[1]
198IDYGKLRKDDPLTNLacetylation[4]
198IDYGKLRKDDPLTNLubiquitination[1]
254HAFSGAQKAETAANRacetylation[2]
312TMHAMQQKLEDFRDYacetylation[2]
312TMHAMQQKLEDFRDYubiquitination[1]
331KPPKVQEKCQLEINFubiquitination[1]
398RLDHLAEKFRQKASIacetylation[2]
398RLDHLAEKFRQKASIubiquitination[1]
402LAEKFRQKASIHEAWubiquitination[1]
420KEAMLRQKDYETATLacetylation[2, 3, 5]
431TATLSEIKALLKKHEacetylation[2]
436EIKALLKKHEAFESDacetylation[2, 3]
436EIKALLKKHEAFESDsuccinylation[3]
633QHNERLRKQFGAQANubiquitination[1]
676SHLRQYEKSIVNYKPacetylation[2]
682EKSIVNYKPKIDQLEacetylation[2, 3]
741QILTRDAKGISQEQMubiquitination[1]
762FNHFDRKKTGMMDTDubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758
Functional Description
  
Sequence Annotation
  
Keyword
 Actin-binding; Calcium; Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 887 AA 
Protein Sequence
MDHYDSQQTN DYMQPEEDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT QIENIEEDFR 60
DGLKLMLLLE VISGERLAKP ERGKMRVHKI SNVNKALDFI ASKGVKLVSI GAEEIVDGNV 120
KMTLGMIWTI ILRFAIQDIS VEETSAKEGL LLWCQRKTAP YKNVNIQNFH ISWKDGLGFC 180
ALIHRHRPEL IDYGKLRKDD PLTNLNTAFD VAERFLDIPK MLDAEDIVGT ARPDEKAIMT 240
YVSSFYHAFS GAQKAETAAN RICKVLAVNQ ENEQLMEDYE KLASDLLEWI RRTIPWLENR 300
VPENTMHAMQ QKLEDFRDYR RLHKPPKVQE KCQLEINFNT LQTKLRLSNR PAFMPSEGRM 360
VSDINNAWGC LEQAEKGYEE WLLNEIRRLE RLDHLAEKFR QKASIHEAWT DGKEAMLRQK 420
DYETATLSEI KALLKKHEAF ESDLAAHQDR VEQIAAIAQE LNELDYYDSP SVNARCQKIC 480
DQWDNLGALT QKRREALERT EKLLETIDQL YLEYAKRAAP FNNWMEGAME DLQDTFIVHT 540
IEEIQGLTTA HEQFKATLPD ADKERLAILG IHNEVSKIVQ TYHVNMAGTN PYTTITPQEI 600
NGKWDHVRQL VPRRDQALTE EHARQQHNER LRKQFGAQAN VIGPWIQTKM EEIGRISIEM 660
HGTLEDQLSH LRQYEKSIVN YKPKIDQLEC DHQLIQEALI FDNKHTNYNM EHIRVGWEQL 720
LTTIARTINE VENQILTRDA KGISQEQMNE FRASFNHFDR KKTGMMDTDD FRACLISMGY 780
NMGEAEFARI MSIVDPNRLG VVTFQAFIDF MSRETADTDT ADQVMASFKI LAGDKNYITE 840
DELRRELPPD QAEYCIARMA PYAGPDSVPG ALDYMSFSTA LYGESDL 887 
Gene Ontology
 GO:0005916; C:fascia adherens; IDA:MGI.
 GO:0030018; C:Z disc; IDA:MGI.
 GO:0051015; F:actin filament binding; IDA:MGI.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0051764; P:actin crosslink formation; IEA:InterPro.
 GO:0051017; P:actin filament bundle assembly; IEA:InterPro. 
Interpro
 IPR001589; Actinin_actin-bd_CS.
 IPR026921; Alpha-actinin.
 IPR001715; CH-domain.
 IPR011992; EF-hand-like_dom.
 IPR014837; EF-hand_Ca_insen.
 IPR002048; EF_hand_dom.
 IPR018159; Spectrin/alpha-actinin.
 IPR002017; Spectrin_repeat. 
Pfam
 PF00307; CH
 PF13405; EF_hand_4
 PF08726; efhand_Ca_insen
 PF00435; Spectrin 
SMART
 SM00033; CH
 SM00054; EFh
 SM00150; SPEC 
PROSITE
 PS00019; ACTININ_1
 PS00020; ACTININ_2
 PS50021; CH
 PS50222; EF_HAND_2 
PRINTS