CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010027
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Glutamate--tRNA ligase 
Protein Synonyms/Alias
 Glutamyl-tRNA synthetase; GluRS 
Gene Name
 gltX 
Gene Synonyms/Alias
 MPN_678; MP164 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
111AYRCFCTKEKLEHERacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 
Functional Description
 Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) (By similarity). 
Sequence Annotation
 MOTIF 10 20 "HIGH" region.
 MOTIF 252 256 "KMSKS" region.
 BINDING 255 255 ATP (By similarity).  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 484 AA 
Protein Sequence
MEKIRTRYAP SPTGYLHVGG ARTAIFNFLL AKHFNGEFII RIEDTDTERN VEGGIESQLE 60
NLRWLGIIPD ESIYNPGNYG PYIQSQKLAT YKKLAYELVG KGLAYRCFCT KEKLEHERQL 120
ALEHHQTPKY LGTCRNLHSK HIQTNLDNQV PFTIRLKINQ DAEFAWNDQV RGKITIPGNS 180
LTDIVLLKAN GIATYNFAVV IDDHDMEITD VLRGAEHISN TAYQLAINQA LGYQRIPRFG 240
HLSVIVDKSG KKLSKRDTKT IQFIEQFKQE GYLPEAVVNF LALLGWNSDF NREFFTINQL 300
IESFTVNRVV GAPAFFDIKK LQWINAHYIK ELSDNAYFNF IDNYLTIDFD YLKNKRKEVS 360
LLFKNQLAFG IEINQLIKET FAPKLGVQHL SVKHRELFKE LQSALQQLSE QLQALPDWTK 420
DNVKSTLTQI GEQFNLKGKK LFMPLRLIFT NKEHGPDLAG IMVLHGKTQV LALLQEFIHA 480
TNLF 484 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004818; F:glutamate-tRNA ligase activity; IEA:HAMAP.
 GO:0000049; F:tRNA binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:HAMAP.
 GO:0006424; P:glutamyl-tRNA aminoacylation; IEA:HAMAP. 
Interpro
 IPR008925; aa-tRNA-synth_I_codon-bd.
 IPR020751; aa-tRNA-synth_I_codon-bd_sub2.
 IPR001412; aa-tRNA-synth_I_CS.
 IPR004527; Glu-tRNA-ligase_Ib_bac/mito.
 IPR000924; Glu/Gln-tRNA-synth_Ib.
 IPR020061; Glu/Gln-tRNA-synth_Ib_a-bdl.
 IPR020058; Glu/Gln-tRNA-synth_Ib_cat-dom.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF00749; tRNA-synt_1c 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR00987; TRNASYNTHGLU.