CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022813
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Epidermal growth factor receptor substrate 15-like 1 
Protein Synonyms/Alias
 Eps15-related protein; Eps15R 
Gene Name
 EPS15L1 
Gene Synonyms/Alias
 EPS15R 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
492QSQESDLKSQEDDLNubiquitination[1, 2, 3]
531VQLETIIKSLKSTQDubiquitination[1, 2, 3, 4, 5, 6]
534ETIIKSLKSTQDEINubiquitination[1, 2, 3]
602GAMDDPFKNKALLFSubiquitination[1, 4, 6]
604MDDPFKNKALLFSNNubiquitination[1, 3, 4, 6]
627FQTEDPFKSDPFKGAubiquitination[1, 3]
632PFKSDPFKGADPFKGubiquitination[1, 4, 6]
638FKGADPFKGDPFQNDubiquitination[1, 3, 4, 6]
664PFGGDPFKESDPFRGubiquitination[1, 3]
679SATDDFFKKQTKNDPubiquitination[3, 4, 6]
683DFFKKQTKNDPFTSDubiquitination[3, 4, 6]
694FTSDPFTKNPSLPSKubiquitination[1, 3, 4, 5]
701KNPSLPSKLDPFESSubiquitination[1, 2, 3, 4, 5, 6]
719SSSSVSSKGSDPFGTubiquitination[3]
828SGDPFQSKKGFGDPFubiquitination[1, 3, 4, 5, 6, 7, 8]
829GDPFQSKKGFGDPFSubiquitination[1, 3, 4, 5, 6, 8]
838FGDPFSGKDPFVPSSubiquitination[1, 3, 4, 5, 6, 9]
848FVPSSAAKPSKASASubiquitination[1, 3, 4, 6, 8, 9]
851SSAAKPSKASASGFAubiquitination[4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2. 
Sequence Annotation
 DOMAIN 15 104 EH 1.
 DOMAIN 127 215 EH 2.
 DOMAIN 159 194 EF-hand.
 DOMAIN 275 365 EH 3.
 REPEAT 618 620 1.
 REPEAT 640 642 2.
 REPEAT 645 647 3.
 REPEAT 650 652 4.
 REPEAT 656 658 5.
 REPEAT 661 663 6.
 REPEAT 667 669 7.
 REPEAT 685 687 8.
 REPEAT 690 692 9.
 REPEAT 709 711 10.
 REPEAT 728 730 11.
 REPEAT 754 756 12.
 REPEAT 806 808 13.
 REPEAT 812 814 14.
 REPEAT 833 835 15.
 REGION 15 368 Interaction with DAB2 (By similarity).
 REGION 618 835 15 X 3 AA repeats of D-P-F.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 9 9 Phosphoserine (By similarity).
 MOD_RES 30 30 Phosphotyrosine (By similarity).
 MOD_RES 31 31 Phosphothreonine (By similarity).
 MOD_RES 74 74 Phosphotyrosine (By similarity).
 MOD_RES 229 229 Phosphoserine.
 MOD_RES 238 238 Phosphoserine (By similarity).
 MOD_RES 244 244 Phosphoserine.
 MOD_RES 250 250 Phosphoserine (By similarity).
 MOD_RES 255 255 Phosphoserine.
 MOD_RES 355 355 Phosphoserine (By similarity).
 MOD_RES 366 366 Phosphothreonine (By similarity).
 MOD_RES 560 560 Phosphoserine.
 MOD_RES 577 577 Phosphothreonine.
 MOD_RES 797 797 Phosphothreonine.  
Keyword
 Acetylation; Alternative splicing; Calcium; Cell membrane; Coated pit; Coiled coil; Complete proteome; Endocytosis; Membrane; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 864 AA 
Protein Sequence
MAAPLIPLSQ QIPTGNSLYE SYYKQVDPAY TGRVGASEAA LFLKKSGLSD IILGKIWDLA 60
DPEGKGFLDK QGFYVALRLV ACAQSGHEVT LSNLNLSMPP PKFHDTSSPL MVTPPSAEAH 120
WAVRVEEKAK FDGIFESLLP INGLLSGDKV KPVLMNSKLP LDVLGRVWDL SDIDKDGHLD 180
RDEFAVAMHL VYRALEKEPV PSALPPSLIP PSKRKKTVFP GAVPVLPASP PPKDSLRSTP 240
SHGSVSSLNS TGSLSPKHSL KQTQPTVNWV VPVADKMRFD EIFLKTDLDL DGYVSGQEVK 300
EIFMHSGLTQ NLLAHIWALA DTRQTGKLSK DQFALAMYFI QQKVSKGIDP PQVLSPDMVP 360
PSERGTPGPD SSGSLGSGEF TGVKELDDIS QEIAQLQREK YSLEQDIREK EEAIRQKTSE 420
VQELQNDLDR ETSSLQELEA QKQDAQDRLD EMDQQKAKLR DMLSDVRQKC QDETQMISSL 480
KTQIQSQESD LKSQEDDLNR AKSELNRLQQ EETQLEQSIQ AGRVQLETII KSLKSTQDEI 540
NQARSKLSQL HESRQEAHRS LEQYDQVLDG AHGASLTDLA NLSEGVSLAE RGSFGAMDDP 600
FKNKALLFSN NTQELHPDPF QTEDPFKSDP FKGADPFKGD PFQNDPFAEQ QTTSTDPFGG 660
DPFKESDPFR GSATDDFFKK QTKNDPFTSD PFTKNPSLPS KLDPFESSDP FSSSSVSSKG 720
SDPFGTLDPF GSGSFNSAEG FADFSQMSKP PPSGPFTSSL GGAGFSDDPF KSKQDTPALP 780
PKKPAPPRPK PPSGKSTPVS QLGSADFPEA PDPFQPLGAD SGDPFQSKKG FGDPFSGKDP 840
FVPSSAAKPS KASASGFADF TSVS 864 
Gene Ontology
 GO:0005905; C:coated pit; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0007173; P:epidermal growth factor receptor signaling pathway; TAS:Reactome.
 GO:0042059; P:negative regulation of epidermal growth factor receptor signaling pathway; TAS:Reactome. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology. 
Pfam
  
SMART
 SM00054; EFh
 SM00027; EH 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH 
PRINTS