CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013939
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myosin-1 
Protein Synonyms/Alias
 Myosin heavy chain 1; Myosin heavy chain 2x; MyHC-2x; Myosin heavy chain, skeletal muscle, adult 1 
Gene Name
 Myh1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
30ERIEAQNKPFDAKSSubiquitination[1]
35QNKPFDAKSSVFVVDubiquitination[1]
44SVFVVDAKESFVKATubiquitination[1]
63EGGKVTAKTEGGTTVubiquitination[1]
73GGTTVTVKDDQVYPMubiquitination[1]
366VMHYGNMKFKQKQREubiquitination[1]
386DGTEVADKAAYLQNLubiquitination[1]
400LNSADLLKALCYPRVubiquitination[1]
641ESGGGGGKKGAKKKGubiquitination[1]
664LFRENLNKLMTNLRSubiquitination[1]
854LKSAETEKEMANMKEubiquitination[1]
865NMKEEFEKAKENLAKubiquitination[1]
917ERCDQLIKTKIQLEAubiquitination[1]
919CDQLIKTKIQLEAKIubiquitination[1]
946INAELTAKKRKLEDEubiquitination[1]
983HATENKVKNLTEEMAubiquitination[1]
998GLDETIAKLTKEKKAubiquitination[1]
1033TLTKAKIKLEQQVDDubiquitination[1]
1049EGSLEQEKKIRMDLEubiquitination[1]
1247SNMEVISKSKGNLEKubiquitination[1]
1254KSKGNLEKMCRTLEDubiquitination[1]
1267EDQVSELKTKEEEQQubiquitination[1]
1301YSRQLDEKDSLVSQLubiquitination[1]
1331RQLEEEIKAKSALAHacetylation[2]
1331RQLEEEIKAKSALAHubiquitination[1]
1417HVEAVNAKCASLEKTubiquitination[1]
1423AKCASLEKTKQRLQNubiquitination[1]
1451AACAALDKKQRNFDKubiquitination[1]
1466ILAEWKQKYEETHAEubiquitination[1]
1479AELEASQKESRSLSTubiquitination[1]
1492STELFKIKNAYEESLubiquitination[1]
1510ETLKRENKNLQQEISubiquitination[1]
1528EQIAEGGKRIHELEKubiquitination[1]
1544KKQIEQEKSELQAALubiquitination[1]
1624DAIRLKKKMEGDLNEubiquitination[1]
1658RNTQGILKDTQLHLDubiquitination[1]
1675LRGQEDLKEQLAMVEubiquitination[1]
1707EQTERSRKIAEQELLubiquitination[1]
1798KNLEQTVKDLQHRLDubiquitination[1]
1813EAEQLALKGGKKQIQubiquitination[1]
1838GEVENEQKRNVEAIKubiquitination[1]
1845KRNVEAIKGLRKHERubiquitination[1]
1877RLQDLVDKLQSKVKAubiquitination[1]
1881LVDKLQSKVKAYKRQubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379] 
Functional Description
 Muscle contraction. 
Sequence Annotation
 DOMAIN 1 787 Myosin head-like.
 DOMAIN 788 817 IQ.
 NP_BIND 179 186 ATP (Potential).
 REGION 662 684 Actin-binding (By similarity).
 REGION 764 778 Actin-binding (By similarity).
 MOD_RES 130 130 N6,N6,N6-trimethyllysine (Potential).
 MOD_RES 955 955 Phosphoserine (By similarity).
 MOD_RES 1206 1206 Phosphoserine (By similarity).
 MOD_RES 1931 1931 Phosphoserine.
 MOD_RES 1936 1936 Phosphothreonine.  
Keyword
 Actin-binding; ATP-binding; Calmodulin-binding; Coiled coil; Complete proteome; Cytoplasm; Methylation; Motor protein; Muscle protein; Myosin; Nucleotide-binding; Phosphoprotein; Reference proteome; Thick filament. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1942 AA 
Protein Sequence
MSSDAEMAVF GEAAPYLRKS EKERIEAQNK PFDAKSSVFV VDAKESFVKA TVQSREGGKV 60
TAKTEGGTTV TVKDDQVYPM NPPKYDKIED MAMMTHLHEP AVLYNLKERY AAWMIYTYSG 120
LFCVTVNPYK WLPVYNAEVV AAYRGKKRQE APPHIFSISD NAYQFMLTDR ENQSILITGE 180
SGAGKTVNTK RVIQYFATIA VTGEKKKEEA TSGKMQGTLE DQIISANPLL EAFGNAKTVR 240
NDNSSRFGKF IRIHFGTTGK LASADIETYL LEKSRVTFQL KAERSYHIFY QIMSNKKPDL 300
IEMLLITTNP YDYAFVSQGE ITVPSIDDQE ELMATDSAID ILGFTSDERV SIYKLTGAVM 360
HYGNMKFKQK QREEQAEPDG TEVADKAAYL QNLNSADLLK ALCYPRVKVG NEYVTKGQTV 420
QQVYNSVGAL AKAVYEKMFL WMVTRINQQL DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC 480
INFTNEKLQQ FFNHHMFVLE QEEYKKEGIE WEFIDFGMDL AACIELIEKP MGIFSILEEE 540
CMFPKATDTS FKNKLYEQHL GKSNNFQKPK PAKGKVEAHF SLVHYAGTVD YNIAGWLDKN 600
KDPLNETVVG LYQKSSMKTL AYLFSGAAAA AEAESGGGGG KKGAKKKGSS FQTVSALFRE 660
NLNKLMTNLR STHPHFVRCI IPNETKTPGA MEHELVLHQL RCNGVLEGIR ICRKGFPSRI 720
LYADFKQRYK VLNASAIPEG QFIDSKKASE KLLGSIDIDH TQYKFGHTKV FFKAGLLGLL 780
EEMRDDKLAQ LITRTQAMCR GYLARVEYQK MVERRESIFC IQYNVRAFMN VKHWPWMKLY 840
FKIKPLLKSA ETEKEMANMK EEFEKAKENL AKAEAKRKEL EEKMVALMQE KNDLQLQVQS 900
EADSLADAEE RCDQLIKTKI QLEAKIKEVT ERAEDEEEIN AELTAKKRKL EDECSELKKD 960
IDDLELTLAK VEKEKHATEN KVKNLTEEMA GLDETIAKLT KEKKALQEAH QQTLDDLQAE 1020
EDKVNTLTKA KIKLEQQVDD LEGSLEQEKK IRMDLERAKR KLEGDLKLAQ ESTMDVENDK 1080
QQLDEKLKKK EFEMSNLQSK IEDEQALGMQ LQKKIKELQA RIEELEEEIE AERASRAKAE 1140
KQRSDLSREL EEISERLEEA GGATSAQIEM NKKREAEFQK MRRDLEEATL QHEATAATLR 1200
KKHADSVAEL GEQIDNLQRV KQKLEKEKSE MKMEIDDLAS NMEVISKSKG NLEKMCRTLE 1260
DQVSELKTKE EEQQRLINEL TAQRGRLQTE SGEYSRQLDE KDSLVSQLSR GKQAFTQQIE 1320
ELKRQLEEEI KAKSALAHAL QSSRHDCDLL REQYEEEQEA KAELQRAMSK ANSEVAQWRT 1380
KYETDAIQRT EELEEAKKKL AQRLQDAEEH VEAVNAKCAS LEKTKQRLQN EVEDLMIDVE 1440
RTNAACAALD KKQRNFDKIL AEWKQKYEET HAELEASQKE SRSLSTELFK IKNAYEESLD 1500
HLETLKRENK NLQQEISDLT EQIAEGGKRI HELEKIKKQI EQEKSELQAA LEEAEASLEH 1560
EEGKILRIQL ELNQVKSEID RKIAEKDEEI DQLKRNHIRV VESMQSTLDA EIRSRNDAIR 1620
LKKKMEGDLN EMEIQLNHSN RMAAEALRNY RNTQGILKDT QLHLDDALRG QEDLKEQLAM 1680
VERRANLLQA EIEELRATLE QTERSRKIAE QELLDASERV QLLHTQNTSL INTKKKLETD 1740
ISQIQGEMED IVQEARNAEE KAKKAITDAA MMAEELKKEQ DTSAHLERMK KNLEQTVKDL 1800
QHRLDEAEQL ALKGGKKQIQ KLEARVRELE GEVENEQKRN VEAIKGLRKH ERRVKELTYQ 1860
TEEDRKNVLR LQDLVDKLQS KVKAYKRQAE EAEEQSNVNL AKFRKIQHEL EEAEERADIA 1920
ESQVNKLRVK SREVHTKIIS EE 1942 
Gene Ontology
 GO:0031672; C:A band; IDA:MGI.
 GO:0005925; C:focal adhesion; IEA:Compara.
 GO:0014704; C:intercalated disc; IDA:MGI.
 GO:0005859; C:muscle myosin complex; ISO:MGI.
 GO:0032982; C:myosin filament; IEA:UniProtKB-KW.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003774; F:motor activity; IEA:InterPro. 
Interpro
 IPR000048; IQ_motif_EF-hand-BS.
 IPR027401; Myosin-like_IQ_dom.
 IPR015650; Myosin_1/23/4/7/8/13/15.
 IPR001609; Myosin_head_motor_dom.
 IPR004009; Myosin_N.
 IPR002928; Myosin_tail.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00063; Myosin_head
 PF02736; Myosin_N
 PF01576; Myosin_tail_1 
SMART
 SM00015; IQ
 SM00242; MYSc 
PROSITE
 PS50096; IQ 
PRINTS
 PR00193; MYOSINHEAVY.