| Tag | Content |
|---|
| CPLM ID | CPLM-005844 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Thiazole synthase |
Protein Synonyms/Alias | |
Gene Name | thiG |
Gene Synonyms/Alias | b3991; JW5549 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 7 | *MLRIADKTFDSHLF | acetylation | [1] | | 43 | QLVTLAMKRVDLRQH | acetylation | [1] | | 95 | ALGTNWLKLEIHPDA | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S. |
Sequence Annotation | REGION 182 183 DXP binding (By similarity).
REGION 204 205 DXP binding (By similarity).
ACT_SITE 95 95 Schiff-base intermediate with DXP (By
BINDING 156 156 DXP; via amide nitrogen (By similarity). |
Keyword | Complete proteome; Cytoplasm; Direct protein sequencing; Reference proteome; Schiff base; Thiamine biosynthesis; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 256 AA |
Protein Sequence | MLRIADKTFD SHLFTGTGKF ASSQLMVEAI RASGSQLVTL AMKRVDLRQH NDAILEPLIA 60
AGVTLLPNTS GAKTAEEAIF AAHLAREALG TNWLKLEIHP DARWLLPDPI ETLKAAETLV 120
QQGFVVLPYC GADPVLCKRL EEVGCAAVMP LGAPIGSNQG LETRAMLEII IQQATVPVVV 180
DAGIGVPSHA AQALEMGADA VLVNTAIAVA DDPVNMAKAF RLAVEAGLLA RQSGPGSRSY 240
FAHATSPLTG FLEASA 256 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0036355; F:2-iminoacetate synthase activity; IEA:InterPro. GO:0016783; F:sulfurtransferase activity; IEA:HAMAP. GO:0009229; P:thiamine diphosphate biosynthetic process; IEA:UniProtKB-UniPathway. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |