CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019323
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Small G protein signaling modulator 3 
Protein Synonyms/Alias
 Merlin-associated protein; RUN and TBC1 domain-containing protein 3; Rab-GTPase-activating protein-like protein; RabGAPLP 
Gene Name
 SGSM3 
Gene Synonyms/Alias
 MAP; RABGAPLP; RUTBC3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
146LSYREIVKNSSNDETubiquitination[1, 2]
157NDETIAAKQIEKDLLubiquitination[1, 2]
161IAAKQIEKDLLRTMPubiquitination[2]
405RRRTQRRKSTITALLubiquitination[2]
422EDDLEALKAKNIKQTubiquitination[1, 2]
452HFQCTDPKNCSVELTubiquitination[2]
486RSHRRRAKALLDFERubiquitination[2]
532LRGWFPAKFVEVLDEubiquitination[1, 2]
542EVLDERSKEYSIAGDubiquitination[1, 2]
569GTLCPALKALFEHGLubiquitination[2]
578LFEHGLKKPSLLGGAubiquitination[2]
615YSRLVLCKTFRLDEDubiquitination[2]
624FRLDEDGKVLTPEELubiquitination[1, 2]
730REAQQPLKEGVRDMLubiquitination[1, 2]
739GVRDMLVKHHLFSWDubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 May play a cooperative role in NF2-mediated growth suppression of cells. 
Sequence Annotation
 DOMAIN 114 305 Rab-GAP TBC.
 DOMAIN 480 539 SH3.
 DOMAIN 555 718 RUN.  
Keyword
 Alternative splicing; Cell cycle; Coiled coil; Complete proteome; Cytoplasm; Growth arrest; Polymorphism; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 749 AA 
Protein Sequence
MSGSHTPACG PFSALTPSIW PQEILAKYTQ KEESAEQPEF YYDEFGFRVY KEEGDEPGSS 60
LLANSPLMED APQRLRWQAH LEFTHNHDVG DLTWDKIAVS LPRSEKLRSL VLAGIPHGMR 120
PQLWMRLSGA LQKKRNSELS YREIVKNSSN DETIAAKQIE KDLLRTMPSN ACFASMGSIG 180
VPRLRRVLRA LAWLYPEIGY CQGTGMVAAC LLLFLEEEDA FWMMSAIIED LLPASYFSTT 240
LLGVQTDQRV LRHLIVQYLP RLDKLLQEHD IELSLITLHW FLTAFASVVD IKLLLRIWDL 300
FFYEGSRVLF QLTLGMLHLK EEELIQSENS ASIFNTLSDI PSQMEDAELL LGVAMRLAGS 360
LTDVAVETQR RKHLAYLIAD QGQLLGAGTL TNLSQVVRRR TQRRKSTITA LLFGEDDLEA 420
LKAKNIKQTE LVADLREAIL RVARHFQCTD PKNCSVELTP DYSMESHQRD HENYVACSRS 480
HRRRAKALLD FERHDDDELG FRKNDIITIV SQKDEHCWVG ELNGLRGWFP AKFVEVLDER 540
SKEYSIAGDD SVTEGVTDLV RGTLCPALKA LFEHGLKKPS LLGGACHPWL FIEEAAGREV 600
ERDFASVYSR LVLCKTFRLD EDGKVLTPEE LLYRAVQSVN VTHDAVHAQM DVKLRSLICV 660
GLNEQVLHLW LEVLCSSLPT VEKWYQPWSF LRSPGWVQIK CELRVLCCFA FSLSQDWELP 720
AKREAQQPLK EGVRDMLVKH HLFSWDVDG 749 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005921; C:gap junction; IEA:Compara.
 GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
 GO:0017137; F:Rab GTPase binding; IDA:UniProtKB.
 GO:0007050; P:cell cycle arrest; IEA:UniProtKB-KW.
 GO:0045732; P:positive regulation of protein catabolic process; IEA:Compara.
 GO:0032851; P:positive regulation of Rab GTPase activity; IEA:GOC.
 GO:0032486; P:Rap protein signal transduction; IEP:UniProtKB. 
Interpro
 IPR000195; Rab-GTPase-TBC_dom.
 IPR004012; Run.
 IPR001452; SH3_domain. 
Pfam
 PF00566; RabGAP-TBC
 PF02759; RUN
 PF00018; SH3_1 
SMART
 SM00593; RUN
 SM00326; SH3
 SM00164; TBC 
PROSITE
 PS50826; RUN
 PS50002; SH3
 PS50086; TBC_RABGAP 
PRINTS